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Application
FIGURE: 1 / 1
CH25H Antibody (PA5-72349) in WB
Western blot analysis of CH25H in various lysates. Samples were incubated with CH25H polyclonal antibody (Product # PA5-72349) using a dilution of 1:1,000-1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Human brain lysate; Lane 2: Mouse heart lysate; Lane 3: Mouse lung lysate. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-72349
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 47-76 amino acids from human CH25H
Conjugate
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2718203
Target Information
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1.14.99.38; Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase; h25OH; m25OH
Gene Aliases: AI462618; C25H; CH25H; m25OH
UniProt ID: (Human) O95992, (Mouse) Q9Z0F5
Entrez Gene ID: (Human) 9023, (Mouse) 12642
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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