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Calreticulin (CALR) Antibody (CF813229) in WB
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CALR-Knockout HeLa cells (KO, Cat# LC831130) were separated by SDS-PAGE and immunoblotted with anti-CALR monoclonal antibody TA813229 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
产品信息
CF813229
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
OTI15F5
抗原
Full length human recombinant protein of human CALR (NP_004334) produced in HEK293T cell.
偶联物
Unconjugated
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 8% trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
基因别名: CALR; Calregulin; cC1qR; CRT; CRTC; HEL-S-99n; RO; SSA
UniProt ID: (Human) P27797, (Mouse) P14211, (Rat) P18418
Entrez Gene ID: (Human) 811, (Mouse) 12317, (Rat) 64202
complement component C1q binding
glycoprotein binding
DNA binding
mRNA binding
integrin binding
iron ion binding
calcium ion binding
protein binding
zinc ion binding
carbohydrate binding
ubiquitin protein ligase binding
peptide binding
hormone binding
protein binding involved in protein folding
poly(A) RNA binding
androgen receptor binding
unfolded protein binding
chaperone binding
metal ion binding
chaperone
negative regulation of transcription from RNA polymerase II promoter
antigen processing and presentation of peptide antigen via MHC class I
peptide antigen assembly with MHC class I protein complex
regulation of transcription, DNA-templated
protein folding
protein export from nucleus
cellular calcium ion homeostasis
receptor-mediated endocytosis
cell cycle arrest
spermatogenesis
positive regulation of cell proliferation
positive regulation of gene expression
negative regulation of translation
protein maturation by protein folding
cortical actin cytoskeleton organization
response to estradiol
negative regulation of intracellular steroid hormone receptor signaling pathway
response to testosterone
protein localization to nucleus
protein folding in endoplasmic reticulum
ATF6-mediated unfolded protein response
regulation of meiotic nuclear division
response to drug
glucocorticoid receptor signaling pathway
regulation of apoptotic process
negative regulation of neuron differentiation
positive regulation of DNA replication
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of retinoic acid receptor signaling pathway
positive regulation of phagocytosis
protein stabilization
sequestering of calcium ion
cardiac muscle cell differentiation
chaperone-mediated protein folding
cellular response to lithium ion
cellular senescence
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of dendritic cell chemotaxis
response to organic substance
cellular response to organic substance
