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Invitrogen
Complement C9 Polyclonal Antibody
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FIGURE: 1 / 6
Complement C9 Antibody (PA5-29093) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Complement C9 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Complement C9 Polyclonal Antibody (Product # PA5 29093) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-29093
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human C9. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: THP-1, BCL-1, human plasma.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive. C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Complement component C9
Gene Aliases: ARMD15; C9; C9D
UniProt ID: (Human) P02748, (Mouse) P06683, (Rat) Q62930
Entrez Gene ID: (Human) 735, (Mouse) 12279, (Rat) 117512
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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