Invitrogen

EGFR Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

25 Published Figures

22 References

View all (185) EGFR antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

EGFR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 38

EGFR Antibody (PA1-1110) in ICC/IF

Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGFR Rabbit Polyclonal antibody (Product # PA1-1110) at 1 µg/mL dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More

EGFR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EGFR Antibody in Immunoprecipitation (IP)

EGFR Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

EGFR Antibody in Western Blot, Immunohistochemistry (WB, IHC)

EGFR Antibody in Immunohistochemistry (IHC)

Product Image Gallery

EGFR Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with EGFR siRNA and decrease of signal was observed in ICC using EGFR Rabbit Polyclonal antibody (Product # PA1-1110). {KD}

Product Details

PA1-1110

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

2 µg

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C L(1198) R V A P Q S S E F I G A(1210) of human EGFR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_326079

Product Specific Information

PA1-1110 detects EGFR from human, non-human primate (monkey), mouse, and rat samples.

PA1-1110 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, and immunofluorescence procedures. By Western blot, this antibody detects ~190 kDa protein representing EGFR from human HeLa cell extract.

The PA1-1110 immunogen is a synthetic peptide corresponding to residues C L(1198) R V A P Q S S E F I G A(1210) of human EGFR.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration.

Signal transduction and targeted therapy

Wang M,Zhang Z,Liu J,Song M,Zhang T,Chen Y,Hu H,Yang P,Li B,Song X,Pang J,Xing Y,Cao Z,Guo W,Yang H,Wang J,Yang J,Wang C

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Immunohistochemistry

Fri May 13 00:00:00 EDT 2022

Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration.

Signal transduction and targeted therapy

Wang M,Zhang Z,Liu J,Song M,Zhang T,Chen Y,Hu H,Yang P,Li B,Song X,Pang J,Xing Y,Cao Z,Guo W,Yang H,Wang J,Yang J,Wang C

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Immunohistochemistry

Fri May 13 00:00:00 EDT 2022

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

PA1-1110 was used in Western Blot to show for the first time that Hrs/Hgs is a master regulator that modulates myogenesis at different levels through the control of trafficking, signaling, and degradation pathways.

Fri Jul 30 00:00:00 EDT 2021

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

Fri Jul 30 00:00:00 EDT 2021

Chlamydia-induced curvature of the host-cell plasma membrane is required for infection.

Proceedings of the National Academy of Sciences of the United States of America

Hänsch S,Spona D,Murra G,Köhrer K,Subtil A,Furtado AR,Lichtenthaler SF,Dislich B,Mölleken K,Hegemann JH

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Immunocytochemistry

Tue Feb 04 00:00:00 EST 2020

The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion.

Molecular and cellular neurosciences

Gireud-Goss M,Reyes S,Tewari R,Patrizz A,Howe MD,Kofler J,Waxham MN,McCullough LD,Bean AJ

PA1-1110 was used in Western Blot to suggest that ESCRT components critical for membrane protein sorting in the endocytic pathway are altered in AD.

Thu Oct 01 00:00:00 EDT 2020

Aspirin inhibits hepatocellular carcinoma cell proliferation in vitro and in vivo via inducing cell cycle arrest and apoptosis.

Oncology reports

Shi T,Fujita K,Gong J,Nakahara M,Iwama H,Liu S,Yoneyama H,Morishita A,Nomura T,Tani J,Takuma K,Tadokoro T,Himoto T,Oura K,Tsutsui K,Kobara H,Masaki T

PA1-1110 was used in Western Blot to investigate the effects of aspirin on hepatocellular carcinoma (HCC) cells using in vitro and in vivo models.

Sat Aug 01 00:00:00 EDT 2020

Growth factor stimulation promotes multivesicular endosome biogenesis by prolonging recruitment of the late-acting ESCRT machinery.

Proceedings of the National Academy of Sciences of the United States of America

Quinney KB,Frankel EB,Shankar R,Kasberg W,Luong P,Audhya A

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Western Blot

Tue Apr 02 00:00:00 EDT 2019

MicroRNA‑128‑b regulates epidermal growth factor receptor expression in non‑small cell lung cancer.

Molecular medicine reports

Li L,Wang D

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Immunohistochemistry

Sun Dec 01 00:00:00 EST 2019

Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis.

The Journal of clinical investigation

Nam SH,Kim D,Lee D,Lee HM,Song DG,Jung JW,Kim JE,Kim HJ,Kwon NH,Jo EK,Kim S,Lee JW

PA1-1110 was used in Western Blotting to demonstrate that the lysyl-tRNA synthetase-dependent metastatic behaviour of colon cancer spheroids within 3D gels requires communication between cellular molecules and extracellular soluble factors and neighbouring cells.

Thu Nov 01 00:00:00 EDT 2018

Loss of EMILIN-1 Enhances Arteriolar Myogenic Tone Through TGF-β (Transforming Growth Factor-β)-Dependent Transactivation of EGFR (Epidermal Growth Factor Receptor) and Is Relevant for Hypertension in Mice and Humans.

Arteriosclerosis, thrombosis, and vascular biology

Carnevale D,Facchinello N,Iodice D,Bizzotto D,Perrotta M,De Stefani D,Pallante F,Carnevale L,Ricciardi F,Cifelli G,Da Ros F,Casaburo M,Fardella S,Bonaldo P,Innocenzi G,Rizzuto R,Braghetta P,Lembo G,Bressan GM

PA1-1110 was used in Immunohistochemistry-immunofluorescence to implicate an unexpected role of the TGF-β-EGFR pathway in hypertension with current translational perspectives.

Mon Oct 01 00:00:00 EDT 2018

Distinct mechanisms enable inward or outward budding from late endosomes/multivesicular bodies.

Experimental cell research

Gireud-Goss M,Reyes S,Wilson M,Farley M,Memarzadeh K,Srinivasan S,Sirisaengtaksin N,Yamashita S,Tsunoda S,Lang FF,Waxham MN,Bean AJ

PA1-1110 was used in Western Blotting to observe that inward and outward budding from the MVB membrane are mechanistically distinct but may be linked, such that inhibition of inward budding triggers a re-routing of cargo from inward to outward budding vesicles, without affecting the number of vesicles that bud outwardly from MVBs.

Thu Nov 01 00:00:00 EDT 2018

Production of amphiregulin and recovery from influenza is greater in males than females.

Biology of sex differences

Vermillion MS,Ursin RL,Kuok DIT,Vom Steeg LG,Wohlgemuth N,Hall OJ,Fink AL,Sasse E,Nelson A,Ndeh R,McGrath-Morrow S,Mitzner W,Chan MCW,Pekosz A,Klein SL

PA1-1110 was used in Immunocytochemistry-immunoflourescence to show that elevated levels of testosterone and amphiregulin, either independently or in combination, improve resilience and contribute to better influenza outcomes in males compared with females.

Tue Jul 17 00:00:00 EDT 2018

Production of amphiregulin and recovery from influenza is greater in males than females.

Biology of sex differences

Vermillion MS,Ursin RL,Kuok DIT,Vom Steeg LG,Wohlgemuth N,Hall OJ,Fink AL,Sasse E,Nelson A,Ndeh R,McGrath-Morrow S,Mitzner W,Chan MCW,Pekosz A,Klein SL

Tue Jul 17 00:00:00 EDT 2018

Inhibitor of apoptosis proteins are required for effective fusion of autophagosomes with lysosomes.

Cell death & disease

Gradzka S,Thomas OS,Kretz O,Haimovici A,Vasilikos L,Wong WW,Häcker G,Gentle IE

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Western Blot

Tue May 01 00:00:00 EDT 2018

Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival.

PLoS pathogens

Mölleken K,Hegemann JH

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Immunofluorescence

Tue Aug 01 00:00:00 EDT 2017

Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival.

PLoS pathogens

Mölleken K,Hegemann JH

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Immunofluorescence

Tue Aug 01 00:00:00 EDT 2017

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion.

International journal of nanomedicine

Wierzbicki M,Jaworski S,Kutwin M,Grodzik M,Strojny B,Kurantowicz N,Zdunek K,Chodun R,Chwalibog A,Sawosz E

Published figure using EGFR polyclonal antibody (Product # PA1-1110) in Western Blot

Fri Jan 26 00:00:00 EST 2018

A genetically encoded alkyne directs palladium-mediated protein labeling on live mammalian cell surface.

ACS chemical biology

Li N,Ramil CP,Lim RK,Lin Q

PA1-1110 was used in western blot to show that a new alkyne amino acid can be site-selectively incorporated into mammalian proteins

Fri Feb 20 00:00:00 EST 2015

Establishment and characterization of rat portal myofibroblast cell lines.

PloS one

Fausther M,Goree JR,Lavoie ÉG,Graham AL,Sévigny J,Dranoff JA

PA1-1110 was used in western blot to characterize two new cell lines with the potential to be used to study portal (myo)fibroblasts

Mon Mar 21 00:00:00 EDT 2016

Establishment and characterization of rat portal myofibroblast cell lines.

PloS one

Fausther M,Goree JR,Lavoie ÉG,Graham AL,Sévigny J,Dranoff JA

PA1-1110 was used in western blot to characterize two new cell lines with the potential to be used to study portal (myo)fibroblasts

Mon Mar 21 00:00:00 EDT 2016

NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.

PloS one

Fausther M,Lavoie EG,Goree JR,Baldini G,Dranoff JA

PA1-1110 was used in western blot to study the association of disease-causing NT5E mutations with intracellular NT5E mistrafficking

Tue Jan 20 00:00:00 EST 2015

Targeting RNA foci in iPSC-derived motor neurons from ALS patients with a C9ORF72 repeat expansion.

Science translational medicine

Sareen D,O'Rourke JG,Meera P,Muhammad AK,Grant S,Simpkinson M,Bell S,Carmona S,Ornelas L,Sahabian A,Gendron T,Petrucelli L,Baughn M,Ravits J,Harms MB,Rigo F,Bennett CF,Otis TS,Svendsen CN,Baloh RH

PA1-1110 was used in western blot to study the accumulation of RNA foci in motor neurons derived from induced pluripotent stem cells obtained from a patient with C9ORF72 repeat ALS

Wed Oct 23 00:00:00 EDT 2013

Cell-free reconstitution of multivesicular body formation and receptor sorting.

Traffic (Copenhagen, Denmark)

Sun W,Vida TA,Sirisaengtaksin N,Merrill SA,Hanson PI,Bean AJ

PA1-1110 was used in western blot to develop a novel cell-free assay to study multivesicular body formation and receptor sorting

Tue Jun 01 00:00:00 EDT 2010

The UIM domain of Hrs couples receptor sorting to vesicle formation.

Journal of cell science

Urbé S,Sachse M,Row PE,Preisinger C,Barr FA,Strous G,Klumperman J,Clague MJ

PA1-1110 was used in immunocytochemistry to investigate the roles of the UIM-domain of Hrs in the sorting of EGFR and vesicle formation

Wed Oct 15 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; EGFR-related peptide; Epidermal growth factor receptor; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone; waved 2

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Gene Aliases: 9030024J15Rik; AI552599; EGFR; ERBB; ErbB-1; ERBB1; Errb1; Errp; HER1; mENA; NISBD2; PIG61; wa-2; wa2; Wa5

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UniProt ID: (Human) P00533, (Mouse) Q01279

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Entrez Gene ID: (Human) 1956, (Mouse) 13649, (Rat) 24329

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Function(s)

chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion nucleotide binding glycoprotein binding protein kinase activity signal transducer activity receptor binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding epidermal growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EGFR Polyclonal Antibody

EGFR Antibody (PA1-1110) in ICC/IF

Product Details

PA1-1110

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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