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Glutaminase Antibody (MA5-49191) in ICC/IF
Immunocytochemistry analysis of Glutaminase in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Glutaminase Monoclonal antibody (Product # MA5-49191) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and in... View More
产品信息
MA5-49191
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
9G6
抗原
E.coli-derived human Glutaminase/GLS recombinant protein (Position: K396-N654).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_3074849
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue. IHC: human gall bladder adenosquamous carcinoma tissue, human appendiceal adenocarcinoma tissue, human liver cancer tissue, human placenta tissue. ICC/IF: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Glutaminase is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing neurotransmitter glutamate, and maintaing acid-based balance in the kidney. There are alternate splicing results in multiple transcript variants in the Glutaminase gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp686O15119; FLJ10358; GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase
基因别名: 6330442B14; AAD20; AI314027; B230365M23Rik; GAC; GAM; GLS; GLS1; Glut; KGA; KIAA0838; RATGLUT
UniProt ID: (Human) O94925, (Mouse) D3Z7P3, (Rat) P13264
Entrez Gene ID: (Human) 2744, (Mouse) 14660, (Rat) 24398
