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IL-9 Antibody (PA5-47584) in Flow
Flow cytometry of IL-9 in Rat splenocytes were treated for 24 hours with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin. Samples were incubated in IL-9 polyclonal antibody (Product # PA5-47584) or control antibody followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
产品信息
PA5-47584
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
已发表种属
Human,
Rat
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant rat IL-9 Gln19-Ala144
偶联物
Unconjugated
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2576225
产品详细信息
In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse IL-9 is observed, and approximately 10% cross-reactivity with recombinant human IL-9 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ILN; Interleukin; interleukin-9; Interleukin9
Entrez Gene ID: (Rat) 116558
