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Ly-49I Antibody (11-5895-82) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or 0.5 µg of Anti-Mouse Ly-49I FITC (right). Samples were pre-incubated with Anti-Mouse CD16/CD32 Purified (Product # 14-0161-82). Total viable cells were used for analysis.
产品信息
11-5895-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
YLI-90
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_465301
产品详细信息
Description: The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor. Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain. This antibody does not bind to cells from BALB/c, SJL or AKR/J mice.
Applications Reported: YLI-90 has been reported for use in flow cytometric analysis.
Applications Tested: The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: 5E6; Killer cell lectin-like receptor 3; killer cell lectin-like receptor subfamily A, member 9; Ly-49; Ly-49c; Ly-49I receptor; Ly49I receptor; Lymphocyte antigen 49c; Nk2.1; T-cell surface glycoprotein Ly-49C
基因别名: Klra3; Ly-49c; Ly49C; Ly49I; LY49I1
UniProt ID: (Mouse) Q64329
Entrez Gene ID: (Mouse) 16640
