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PSA Antibody (PA1-38514) in ICC/IF
Immunofluorescence analysis of PSA was performed using 70% confluent log phase LNCaP and PC-3 cells treated with Protein transport inhibitor (1X for 4 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSA Polyclonal Antibody (Product # PA1-38514) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Ads... View More
产品信息
PA1-38514
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified human PSA.
偶联物
形式
Liquid
浓度
8.75 mg/mL
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1086868
产品详细信息
A suggested positive control is normal prostate or prostate carcinoma.
靶标信息
PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and is abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. PSA is used to confirm prostatic acinar cell origin in primary and metastatic carcinoma and to rule out non-prostatic carcinoma mimics.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
