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Nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.
Double-stranded RNA (dsRNA) is a molecule composed of two complementary strands of RNA nucleotides arranged in a double helix structure. It plays a diverse role in various biological processes such as gene regulation, RNA interference, and viral defense mechanisms. It can also be used as a tool for studying gene function and as a potential therapeutic agent for treating diseases. However, it is important to note that dsRNA can trigger an immune response in some organisms, which can limit its use in certain applications.
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