Search Thermo Fisher Scientific
The Human Orexin A (OXA) ELISA quantitates OXA in serum, plasma and other biological fluids
原理方法:
The Competitive Orexin A (OXA) ELISA research-use-only kit is designed to quantitatively measure OXA in Humans. An OXA standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human OXA is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human OXA in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : HCRT, NRCLP1, OX, PPORX, PPOX
基因ID : (Human) 3060
基因符号 : HCRT
蛋白别名 : Hcrt, Hypocretin, hypocretin (orexin) neuropeptide, Hypocretin neuropeptide precursor, Orexin precursor, Prepro-orexin, Preprohypocretin
UniProt ID (Human) O43612