Real-time PCR Master Mixes for Gene Expression Analysis

Real-time PCR master mixes for gene expression analysis

Robust gene expression analysis requires consistent, linear amplification of targets across a wide range of concentrations. Because target concentrations vary, it is important to use a qPCR master mix with a wide dynamic range to help ensure you don’t miss detectable changes in expression levels. Applied Biosystems real-time PCR master mixes for gene expression analysis are specifically formulated to efficiently detect targets at both high and very low concentrations, enabling you to capture as much data as possible. View our gene expression master mix product recommendations below.

Which qPCR master mix is right for you?

	TaqMan Fast Advanced Master Mix	TaqMan Fast Virus 1-Step master mixes	PowerTrack SYBR Green Master Mix*
	Check price or order now	Check price or order now	Check price or order now
Key application	Gene expression analysis	Gene expression analysis	Gene expression analysis
Chemistry	2-step probe-based	1-step probe-based	2-step SYBR Green-based
Target	cDNA	RNA	cDNA
Concentration	2X	4X	2X
Multiplexing and passive reference dye	With ROX: up to 2 targets	Available in 2 versions: With ROX: up to 3 targets No ROX: 3 or more targets	With ROX: 1 target
Cycling mode/run time	Fast: ~40 min Standard: ~50 min	Fast: ~60 min Standard: ~90 min	Fast: ~30 min Standard: ~60 min
Carryover contamination control	Available in 2 versions: With UNG No UNG	No UNG	With UNG
Reverse transcriptase	Purchase SuperScript IV VILO Master Mix separately and use before qPCR	Thermostable MMLV RT included in master mix	Purchase SuperScript IV VILO Master Mix separately and use before qPCR
Assay recommendations	TaqMan Gene Expression assays	TaqMan Gene Expression assays	Sequence detection primers
	Request a sample	Request a sample	Request a sample

* For SYBR Green detection: when you need up to 72 hours of benchtop stability, choose PowerUp SYBR Green Master Mix in combination with High-Capacity RNA-to-cDNA Kit Reverse Transcriptase.

TaqPath master mixes*

Applied Biosystems TaqPath general purpose reagents (using TaqMan chemistry) are designed for use in assay development, biopharma development, manufacturing, and QC, and other applications requiring reagents manufactured under current good manufacturing practices (cGMP) and compliance documentation.

Explore TaqPath master mix products for gene expression analysis

For Research Use Only. Not for use in diagnostic procedures.

*For Laboratory Use.