Ion Torrent™ 新一代测序的进步使研究人员能够充分发挥高通量、高准确性和长读取的优势，利用简化的样品制备方法和简单的优化数据分析工作流程，对微生物进行快速准确的测序。在对来自疾病监测、疫情爆发调查以及病因确定研究的存档样品进行研究时，Ion Torrent 测序能够大大加快获得结果的速度。全基因组微生物测序的下游数据分析方法包括 从头 和基于参考序列组装以及微生物菌株分型。
在对一组特定基因（如 16S rRNA）进行靶向测序时，Ion AmpliSeq™ 技术能够提供简单快速的文库构建。该技术基于超高多重 PCR，仅需低至 1 ng 的起始 DNA 量。研究人员利用靶向测序，能够从混合群体中高效鉴别出微生物而无需培养样品，对回顾性疫情爆发样品进行研究，并发现可能与抗生物耐药相关的突变。
Automated and fast template preparation and sequencing of 400 to 600 base-read libraries using the Ion Chef™ System and the Ion S5™ and Ion S5™ XL Sequencing Systems is enabled using the Ion 520™ & Ion 530™ ExT Kit-Chef.
For targeted sequencing of a specific set of genes, such as 16S rRNA, Ion AmpliSeq™ technology delivers simple and fast library construction. Based on ultrahigh-multiplex PCR, Ion AmpliSeq technology requires as little as 1 ng of input DNA. Through the use of targeted sequencing, researchers can efficiently identify the microbes within a mixed population without the need to culture samples, perform research on retrospective outbreak samples, and discover mutations that may be associated with antibiotic resistance.
如今，几个小时的时间就能改变一切。与 Ion PGM 系统的其他模板制备工作流程相比，Ion PGM™ Template IA Kit 有助于将模板制备所需时间缩短一半，实现最快的微生物测序工作流程。
The Evolution of Metagenomic Sequencing
Hear how researchers and researchers are using the Ion 16S™ Metagenomics Kit with Ion Reporter™ Software to make their own discoveries from the trillions of bacterial cells and thousands of species that inhabit our bodies and the environment.
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微生物的多样性
经过数十年的分析和数百项研究，我们发现微生物的多样性远远超出了我们的想象。根据这些物种的多样性，我们能够判断出一个生态系统的健康状态，或者它们的相互作用与炎症性肠道疾病、肥胖和难治性创面等疾病有哪些相关性。请听一听 George Watts 博士和 Charles Li 博士如何将 Ion 16S™ 测序应用于临床和环境研究中。
