Search Thermo Fisher Scientific
脾细胞（b 细胞 t2）
| Organism Type | Mouse | T2 B cells were purified from splenic B cells by cell sorting. Splenic B cells were prepared as described previously. Then 5 x 107 splenic B cells were stained with FITC-conjugated anti-CD21, PE-conjugated anti-HSA, and biotinylated anti-CD23 combined with streptavidin-CyChrome. T2 B cells (CD21hiHSAhiCD23+B220+) were sorted. Staining with anti-CD21, anti-HSA, and anti-CD23 instead of anti-IgM and anti-IgD was performed to avoid contamination of marginal zone B cells and also in order to prevent potential activation signals through the BCR. |
| Tissues | Spleen | |
| Phenotype | Suspension | |
| Primary | yes | |
| Application | Protein Expression |
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