Proteintech

14-3-3 sigma Monoclonal Antibody (3F10D9)

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查看其他28个14-3-3 sigma抗体

抗体检测数据 (10)

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF)

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF)

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14-3-3 sigma Antibody (66251-1-IG) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 66251-1-IG (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF)

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 sigma Antibody in Western Blot (WB)

14-3-3 sigma Antibody in Western Blot (WB)

14-3-3 sigma Antibody in Western Blot (WB)

14-3-3 sigma Antibody in Western Blot (WB)

14-3-3 sigma Antibody in Flow Cytometry (Flow)

Product Image Gallery

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells using 66251-1-IG (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin tissue slide using 66251-1-IG ( 14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens).

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin tissue slide using 66251-1-IG ( 14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens).

14-3-3 sigma Antibody in Western Blot (WB) — MCF-7 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

14-3-3 sigma Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

14-3-3 sigma Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

14-3-3 sigma Antibody in Western Blot (WB) — Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-IG (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

14-3-3 sigma Antibody in Flow Cytometry (Flow) — 1X10^6 A431 cells were intracellularly stained with 0.2 µg Anti-Human 14-3-3 Sigma (Product # 66251-1-IG, Clone:3F10D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.

产品信息

66251-1-IG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:14,000

-

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:8000

-

免疫细胞化学 (ICC/IF)

1:20-1:200

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

-

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3F10D9

抗原

14-3-3 sigma Fusion Protein Ag12644 (1-248 aa encoded by BC002995)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.37 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: MERASLIQK AKLAEQAERY EDMAAFMKGA VEKGEELSCE ERNLLSVAYK NVVGGQRAAW RVLSSIEQKS NEEGSEEKGP EVREYREKVE TELQGVCDTV LGLLDSHLIK EAGDAESRVF YLKMKGDYYR YLAEVATGDD KKRIIDSARS AYQEAMDISK KEMPPTNPIR LGLALNFSVF HYEIANSPEE AISLAKTTFD EAMADLHTLS EDSYKDSTLI MQLLRDNLTL WTADNAGEEG GEAPQEPQS (1-248 aa encoded by BC002995 )

靶标信息

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 14-3-3 protein sigma; 14-3-3 sigma; 14-3-3sigma; Epithelial cell marker protein 1; HGNC:10773; Stratifin

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基因别名: HME1; SFN; YWHAS

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UniProt ID: (Human) P31947

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Entrez Gene ID: (Human) 2810

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protein binding protein kinase C inhibitor activity protein kinase binding protein domain specific binding phosphoprotein binding cadherin binding involved in cell-cell adhesion scaffold/adaptor protein

参与通路

regulation of cyclin-dependent protein serine/threonine kinase activity release of cytochrome c from mitochondria keratinocyte development negative regulation of protein kinase activity DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest signal transduction intrinsic apoptotic signaling pathway in response to DNA damage regulation of epidermal cell division negative regulation of keratinocyte proliferation positive regulation of cell growth keratinization negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of epidermal cell differentiation positive regulation of protein export from nucleus membrane organization establishment of skin barrier negative regulation of protein serine/threonine kinase activity cell-cell adhesion positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway