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5-hydroxymethylcytosine Antibody (MA5-38433) in MeDIP
Methylated DNA immunoprecipitation analysis of 5-hydroxymethylcytosine in 1 µg fragmented human genomic DNA using 0.2 µg of a 5-Methylcytosine monoclonal antibody (Product # MA5-38433). A mouse IgG isotype antibody was used as a negative control. DNA fragments of 300-500 bp from 1 μg of human Hela cells were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The immunoprecipitated ... View More
产品信息
MA5-38433
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
DTA1002
抗原
5-Hydroxymethylcytosine nucleotide
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2898347
靶标信息
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2
