Invitrogen

53BP1 Polyclonal Antibody, DyLight™ 488

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

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53BP1 Antibody (PA5-22754) in WB

Western Blot was performed using Anti-53BP1 Polyclonal Antibody, DyLight 488 (Product # PA5-22754) and a ~270 kDa band corresponding to TP53-binding protein 1 was observed across cell lines tested . Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MDA-MB-231 (Lane 3), U-2 OS (Lane 4), K-562 (Lane 5), U-87 MG (Lane 6) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blottin... View More

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53BP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TP53-binding protein 1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-53BP1 Polyclonal Antibody, DyLight 488 (Product # PA5-22754). {KD}

产品信息

PA5-22754

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

1:100-1:500

流式细胞分析 (Flow)

1.5 µg/mL

产品规格

种属反应

Bat, Bovine, Dog, Fish, Goat, Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The epitope recognized by this antibody maps to a region between residues 350 and 400 of human tumor protein p53 binding protein 1 using the numbering given in entry NP_005648.1.

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偶联物

DyLight™ 488 DyLight™ 488 DyLight™ 488

激发/发射光谱

492/519 nm 查看光谱

形式

Liquid

浓度

0.55 mg/mL

纯化类型

Antigen affinity chromatography

保存液

50mM sodium borate

内含物

0.05% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11155998

产品详细信息

Human has been tested in WB, IHC-P and ICC/IF, mouse and goat have only been tested in ICC/IF.

Suggested positive control: U205, 293T, MO59K cell lysates and MEF lysates .

靶标信息

53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 53BP1; FLJ41424; MGC138366; murine p53-binding protein; p53-binding protein 1; TP53-binding protein 1; transformation related protein 53 binding protein 1; transformation related protein 53 binding-protein 1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1

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基因别名: 53BP1; m53BP1; p202; p53BP1; TDRD30; TP53; TP53BP1; Trp53bp1

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UniProt ID: (Human) Q12888, (Mouse) P70399