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ABCB4 Antibody (PA5-100194) in ICC/IF
Immunocytochemistry analysis of ABCB4 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ABCB4 polyclonal antibody (Product # PA5-100194) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100194
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ABCB4(Accession P21439), corresponding to amino acid residues C353-Y403.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2815724
产品详细信息
Antibody detects endogenous levels of total MDR3.
靶标信息
The membrane-associated protein encoded ABCB4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ABC20; ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; CLCS; GP170; MDR1; MGC163296; Multidrug resistance protein 2; Multidrug resistance protein 3; multiple drug resistance 3; P glycoprotein 2; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein 2; P-glycoprotein 3; P-glycoprotein-3/multiple drug resistance-3; P-GP; PGY1; Phosphatidylcholine translocator ABCB4
基因别名: ABC21; ABCB4; GBD1; ICP3; mdr-2; MDR2; MDR2/3; MDR3; PFIC-3; Pgy-2; Pgy2; PGY3
UniProt ID: (Human) P21439, (Mouse) P21440
Entrez Gene ID: (Human) 5244, (Mouse) 18670
protein binding
ATP binding
phospholipid transporter activity
phosphatidylcholine transporter activity
xenobiotic-transporting ATPase activity
ATPase activity, coupled to transmembrane movement of substances
phosphatidylcholine-translocating ATPase activity
nucleotide binding
hydrolase activity
ATPase activity
ATP-binding cassette (ABC) transporter
antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent
positive regulation of antigen processing and presentation of peptide antigen via MHC class I
lipid metabolic process
transport
drug transmembrane transport
positive regulation of cholesterol transport
bile acid secretion
response to drug
xenobiotic transport
phospholipid translocation
transmembrane transport
lipid homeostasis
positive regulation of phospholipid translocation
response to fenofibrate
cellular response to bile acid
positive regulation of phospholipid transport
