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ACAD9 Antibody (PA5-103559) in ICC/IF
Immunocytochemistry analysis of ACAD9 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ACAD9 polyclonal antibody (Product # PA5-103559) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103559
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ACAD9(Accession Q9H845), corresponding to amino acid residues L348-L398.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2852895
产品详细信息
Antibody detects endogenous levels of total ACAD9.
靶标信息
This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ACAD-9; Acyl-CoA dehydrogenase family member 9; acyl-CoA dehydrogenase family member 9, mitochondrial; acyl-CoA dehydrogenase family, member 9; acyl-Coenzyme A dehydrogenase family, member 9; Complex I assembly factor ACAD9, mitochondrial; very-long-chain acyl-CoA dehydrogenase VLCAD; very-long-chain acyl-CoA dehydrogenase VLCAD homolog
基因别名: 2600017P15Rik; 4732402K02; ACAD9; C630012L17Rik; NPD002; Nyggf2
UniProt ID: (Human) Q9H845, (Rat) B1WC61, (Mouse) Q8JZN5
Entrez Gene ID: (Human) 28976, (Rat) 294973, (Mouse) 229211
fatty-acyl-CoA binding
acyl-CoA dehydrogenase activity
protein binding
electron carrier activity
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
molecular_function
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
short-branched-chain-acyl-CoA dehydrogenase activity
very-long-chain-acyl-CoA dehydrogenase activity
4-methyloctanoyl-CoA dehydrogenase activity
naphthyl-2-methyl-succinyl-CoA dehydrogenase activity
2-methylhexanoyl-CoA dehydrogenase activity
propionyl-CoA dehydrogenase activity
thiol-driven fumarate reductase activity
coenzyme F420-dependent 2,4,6-trinitrophenol reductase activity
coenzyme F420-dependent 2,4,6-trinitrophenol hydride reductase activity
coenzyme F420-dependent 2,4-dinitrophenol reductase activity
medium-chain-acyl-CoA dehydrogenase activity
dehydrogenase
