Invitrogen

ADAMTS5 Polyclonal Antibody

ADAMTS5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ADAMTS5 Antibody (PA5-27165) in IHC (P)

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ADAMTS5 (Product # PA5-27165) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

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产品信息

PA5-27165

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Human disintegrin and metalloproteinase with thrombospondin motifs 5

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544641

产品详细信息

Recommended positive controls: HCT116, MCF-7, JC.

Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ADAMTS5, also known as Implantin or Aggrecanase 2, is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin-5 motif) was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Synergetic enrichment of aggrecan in nucleus pulposus cells by scAAV6-shRNA-mediated knockdown of aggrecanase-1 and aggrecanase-2.

Experimental biology and medicine (Maywood, N.J.)

Mern DS,Thomé C

Published figure using ADAMTS5 polyclonal antibody (Product # PA5-27165) in Western Blot

Sat Jul 01 00:00:00 EDT 2023

Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression.

Science advances

Zhang H,Wang L,Cui J,Wang S,Han Y,Shao H,Wang C,Hu Y,Li X,Zhou Q,Guo J,Zhuang X,Sheng S,Zhang T,Zhou D,Chen J,Wang F,Gao Q,Jing Y,Chen X,Su J

PA5-27165 was used in Immunohistochemistry-immunofluorescence to show that Oroxylin A, an Lcp1-encoded protein l-plastin (LPL) inhibitor, could alleviate OA progression.

Wed Apr 05 00:00:00 EDT 2023

Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration.

Oxidative medicine and cellular longevity

Yu XJ,Liu QK,Lu R,Wang SX,Xu HR,Wang YG,Bao Y,Jiang YQ,Li MW,Kang H

PA5-27165 was used in Immunohistochemistry to study the mechanism underlying the effects of BMSC-derived extracellular vesicles (BMSC-EVs) on nucleus pulposus cells (NPCs) in intervertebral disc degeneration.

Tue Jul 19 00:00:00 EDT 2022

Downregulation of MicroRNA-495 Alleviates IL-1β Responses among Chondrocytes by Preventing SOX9 Reduction.

Yonsei medical journal

Joung S,Yoon DS,Cho S,Ko EA,Lee KM,Park KH,Lee JW,Kim SH

PA5-27165 was used in Western Blotting to investigate whether miRNA-495-mediated SOX9 regulation could be a novel therapeutic target for osteoarthritis using an in vitro cell culture model.

Thu Jul 01 00:00:00 EDT 2021

Downregulation of MicroRNA-495 Alleviates IL-1β Responses among Chondrocytes by Preventing SOX9 Reduction.

Yonsei medical journal

Joung S,Yoon DS,Cho S,Ko EA,Lee KM,Park KH,Lee JW,Kim SH

PA5-27165 was used in Western Blotting to investigate whether miRNA-495-mediated SOX9 regulation could be a novel therapeutic target for osteoarthritis using an in vitro cell culture model.

Thu Jul 01 00:00:00 EDT 2021

Galnt1 is required for normal heart valve development and cardiac function.

PloS one

Tian E,Stevens SR,Guan Y,Springer DA,Anderson SA,Starost MF,Patel V,Ten Hagen KG,Tabak LA

PA5-27165 was used in immunohistochemistry - paraffin section to study heart valve development and cardiac function in Galnt1 KO mice

Tue May 03 00:00:00 EDT 2016

生物信息学

蛋白别名: a disintegrin and metalloproteinase; A disintegrin and metalloproteinase with thrombospondin motifs 11; A disintegrin and metalloproteinase with thrombospondin motifs 5; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); ADAM; ADAM-TS 11; ADAM-TS 5; ADAMs; ADAMTS-5; ADMP-2; Aggrecanase 2; Aggrecanase-2; Implantin; metalloendopeptidases

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基因别名: 9530092O11Rik; ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11; ADAMTS-5; ADAMTS1; ADAMTS11; ADAMTS5; ADMP-2; ADMP2; AI481094; ASMP-2

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UniProt ID: (Human) Q9UNA0, (Mouse) Q9R001

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Entrez Gene ID: (Human) 11096, (Mouse) 23794

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功能