OriGene

ADH5 Monoclonal Antibody (OTI10A10), TrueMAB™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他16个ADH5抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

ADH5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ADH5 Antibody (CF809490) in IHC (P)

Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-ADH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809490)(1:150).

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ADH5 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in ADH5 knockout (KO) HeLa cell line using Anti-ADH5 Monoclonal Antibody (OTI10A10), TrueMAB™ (Product # CF809490). {KO}

产品信息

CF809490

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化（石蜡） (IHC (P))

1:150

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI10A10

抗原

Human recombinant protein fragment corresponding to amino acids 1-266 of human ADH5 produced in E.coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ADH-B2; alcohol dehydrogenase (class III), chi polypeptide; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; class III alcohol dehydrogenase, chi subunit; epididymis secretory sperm binding protein Li 60p; FALDH; FDH; formaldehyde dehydrogenase; Glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase

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基因别名: Adh-2; ADH-3; Adh-5; Adh2; Adh3; ADH5; ADHX; FALDH; FDH; GSH-FDH; GSNOR; HEL-S-60p

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UniProt ID: (Human) P11766, (Rat) P12711, (Mouse) P28474

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Entrez Gene ID: (Human) 128, (Rat) 100145871, (Mouse) 11532

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