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AF9 Antibody (PA5-27797) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of AF9 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
产品信息
PA5-27797
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 281 and 532 of Human AF9
偶联物
形式
Liquid
浓度
0.46 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545273
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2, THP-1, MLLT3 shRNA-transfected 293T, SEM-K2.
Predicted reactivity: Mouse (98%), Rat (98%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AF-9 protein; ALL1-fused gene from chromosome 9 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; myeloid/lymphoid or mixed-lineage leukemia; translocated to 3; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 3; Protein AF-9; YEATS domain-containing protein 3
基因别名: AF9; MLLT3; YEATS3
UniProt ID: (Human) P42568
Entrez Gene ID: (Human) 4300
