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Invitrogen
AFP Monoclonal Antibody (AFP-01)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 5
AFP Antibody (MA1-19178) in ICC/IF
Immunofluorescence analysis of AFP was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with AFP Mouse Monoclonal Antibody (Product # MA1-19178) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilu... View More
产品信息
MA1-19178
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
AFP-01
抗原
Purified alpha Fetoprotein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1070198
产品详细信息
This antibody reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus. This antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MA1-19178 (clone AFP-01).
Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions; Western Blot: non-reducing conditions.
靶标信息
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: a fetoglobulin; a fetoprotein; Alpha fetoglobulin; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; fetoglobulin; fetoprotein; α fetoglobulin; α fetoprotein
基因别名: AFP; AFPD; FETA; HPAFP
UniProt ID: (Human) P02771
Entrez Gene ID: (Human) 174
