Invitrogen

AHR Recombinant Rabbit Monoclonal Antibody (BLR118H)

产品信息

MA5-44427

应用

建议稀释比

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免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:100-1:500

免疫沉淀 (IP)

20 µL/1 mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

BLR118H

抗原

Peptide representing a region between residue 798 and the C-terminus (residue 848) of Human aryl hydrocarbon receptor.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

100 µg/mL

纯化类型

purified

保存液

BBS, pH 8.2, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Wet ice

RRID

AB_2926557

产品详细信息

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

靶标信息

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。