Invitrogen

AIF Monoclonal Antibody (7F7AB10)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

AIF Antibody in Immunocytochemistry (ICC/IF)

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AIF Antibody (45-6200) in ICC/IF

Immunofluorescence analysis of AIF was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with AIF Monoclonal Antibody (7F7AB10) (Product # 45-6200) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A281... View More

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AIF Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with AIF siRNA and decrease in signal intensity was observed in Western Blot application using Anti-AIF Monoclonal Antibody (7F7AB10) (Product # 45-6200). {KD}

产品信息

45-6200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-5 µg/mL

免疫组化 (IHC)

10 µg/mL

免疫细胞化学 (ICC/IF)

5-10 µg/mL

流式细胞分析 (Flow)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

7F7AB10

抗原

Human heart mitochondria

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

运输条件

Wet ice

RRID

AB_2533822

产品详细信息

When performing IHC use heat mediated antigen retrieval before commencing with IHC staining protocol.

When perfoming ICC or IF recommended sample treatment are paraformaldehyde fixed at 4% for 20 minutes and Triton X-100 permeabilized at 0.1% for 15 minutes.

靶标信息

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ERP18; ERP19; hAG-1; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376

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基因别名: AIF; AIFM1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8

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UniProt ID: (Human) O95831

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Entrez Gene ID: (Human) 9131

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功能

DNA binding protein binding electron carrier activity NAD(P)H oxidase activity oxidoreductase activity, acting on NAD(P)H protein dimerization activity FAD binding oxidoreductase

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