Invitrogen

ALKBH5 Recombinant Rabbit Monoclonal Antibody (10H24L9)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 4

ALKBH5 Antibody (703570) in WB

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), MDA-MB-231 (Lane 4), IMR-32 (Lane 5) and tissue extract of Mouse Testis (Lane 6). The blot was probed with Anti-ALKBH5 Recombinant Rabbit Monoclonal Antibody (Product # 703570, 1:5,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A ~44 kDa band corresponding to ALKB... View More

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产品信息

703570

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

10H24L9

抗原

Protein corresponding to human ALKBH5 [aa66-aa292]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2762417

产品详细信息

This antibody is predicted to react with Monkey, Cat, Pig.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

ALKBH5 (RNA demehtylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation. Specifically, it demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylation of m6A mRNA affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). It requires molecular oxygen, alpha-ketoglutarate and iron.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

K235 acetylation couples with PSPC1 to regulate the m⁶A demethylation activity of ALKBH5 and tumorigenesis.

Nature communications

Zhang XL,Chen XH,Xu B,Chen M,Zhu S,Meng N,Wang JZ,Zhu H,Chen D,Liu JB,Yan GR

Published figure using ALKBH5 recombinant monoclonal antibody (Product # 703570) in Western Blot

Tue Jun 27 00:00:00 EDT 2023

Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response.

Nucleic acids research

Yu F,Wei J,Cui X,Yu C,Ni W,Bungert J,Wu L,He C,Qian Z

703570 was used in Western Blot to uncover a molecular mechanism involving ALKBH5 PTMs and increased mRNA m6A levels that protect genomic integrity of cells in response to ROS.

Fri Jun 04 00:00:00 EDT 2021

生物信息学

蛋白别名: AlkB family member 5, RNA demethylase; alkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; RNA demethylase ALKBH5

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基因别名: ABH5; ALKBH5; AW050020; AW215868; E130207K11; OFOXD; OFOXD1

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UniProt ID: (Human) Q6P6C2, (Mouse) Q3TSG4

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Entrez Gene ID: (Human) 54890, (Mouse) 268420

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功能