Invitrogen

ALOX15 Monoclonal Antibody (OTI3G8)

ALOX15 Antibody in Immunocytochemistry (ICC/IF)

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ALOX15 Antibody (MA5-25853) in ICC/IF

Immunofluorescent analysis of ALOX15 in COS7 cells. Cells were transfected with a plasmid overexpressing ALOX15 and probed with a ALOX15 monoclonal antibody (Product # MA5-25853).

ALOX15 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-25853

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化（石蜡） (IHC (P))

1:150

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

OTI3G8

抗原

Full length human recombinant protein of ALOX15 produced in HEK293T cell

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2722848

靶标信息

ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Paradoxical Induction of ALOX15/15B by Cortisol in Human Amnion Fibroblasts: Implications for Inflammatory Responses of the Fetal Membranes at Parturition.

International journal of molecular sciences

Zhang F,Lu JW,Lei WJ,Li MD,Pan F,Lin YK,Wang WS,Sun K

MA5-25853 was used in Western Blotting to examine whether glucocorticoids could induce ALOX15/15B expression and 15(S)-HETE production to promote PGE2 synthesis in amnion fibroblasts at parturition.

Thu Jun 29 00:00:00 EDT 2023

Paradoxical Induction of ALOX15/15B by Cortisol in Human Amnion Fibroblasts: Implications for Inflammatory Responses of the Fetal Membranes at Parturition.

International journal of molecular sciences

Zhang F,Lu JW,Lei WJ,Li MD,Pan F,Lin YK,Wang WS,Sun K

MA5-25853 was used in Western Blotting to examine whether glucocorticoids could induce ALOX15/15B expression and 15(S)-HETE production to promote PGE2 synthesis in amnion fibroblasts at parturition.

Thu Jun 29 00:00:00 EDT 2023

Growth State-Dependent Expression of Arachidonate Lipoxygenases in the Human Endothelial Cell Line EA.hy926.

Cells

Sabbir MG,Wigle JT,Taylor CG,Zahradka P

MA5-25853 was used in Western Blotting to show that the presence of ALOX15B and ALOXE3 exclusively in quiescent human endothelial cells suggests their activity likely contributes to the maintenance of a healthy endothelium.

Wed Aug 10 00:00:00 EDT 2022

Growth State-Dependent Expression of Arachidonate Lipoxygenases in the Human Endothelial Cell Line EA.hy926.

Cells

Sabbir MG,Wigle JT,Taylor CG,Zahradka P

Wed Aug 10 00:00:00 EDT 2022

Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition.

Journal of lipid research

Zhang F,Sun K,Wang WS

MA5-25853 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to conclude that 15(S)-HETE is identified as another crucial parturition-pertinent AA-derived eicosanoid in the amnion, which may form a feed-forward loop with PGE2 in parturition.

Tue Nov 01 00:00:00 EDT 2022

生物信息学

蛋白别名: 12-LOX; 12/15-lipoxygenase; 12/15-LOX; 15-lipooxygenase-1; 15-LOX; 15-LOX2; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; Polyunsaturated fatty acid lipoxygenase ALOX15

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基因别名: 12-LOX; 15-LOX; 15-LOX-1; 15LOX-1; Alox12; Alox12l; ALOX15; LOG15

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UniProt ID: (Human) P16050, (Rat) Q02759

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Entrez Gene ID: (Human) 246, (Rat) 81639

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功能

arachidonate 12-lipoxygenase activity iron ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding hepoxilin-epoxide hydrolase activity arachidonate 15-lipoxygenase activity hepoxilin A3 synthase activity eoxin A4 synthase activity potassium channel inhibitor activity oxygenase

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