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AMIGO1 Antibody (MA5-45619) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with AMIGO-1 monoclonal antibody (Product # MA5-45619) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red ... View More
产品信息
MA5-45619
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
L86/33
抗原
Fusion proteinamino acids 394-492 (cytoplasmic C-terminus) of mouse AMIGO-1
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932073
产品详细信息
1 µg/mL of MA5-45619 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 60-80kDa depending on maturity/glycosylation.
This antibody was formerly sold as clone S86-36.
靶标信息
Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: adhesion molecule with Ig-like domain 1; alivin 2; Alivin-2; AMIGO 1; AMIGO-1; AMIGO1; amigo1 {ECO:0000250|UniProtKB:Q80ZD8}; amphoretin-induced gene and ORF; amphoterin induced gene and ORF (Amigo); amphoterin-induced gene and ORF; Amphoterin-induced protein 1; MGC25558; OTTHUMP00000013379; RP23 89M15.6; transmembrane protein AMIGO
基因别名: ALI2; AMIGO; AMIGO-1; AMIGO1; KIAA1163
UniProt ID: (Human) Q86WK6, (Mouse) Q80ZD8, (Rat) Q80ZD7
Entrez Gene ID: (Human) 57463, (Mouse) 229715, (Rat) 295365
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
multicellular organism development
nervous system development
axonogenesis
axonal fasciculation
brain development
cell differentiation
myelination
positive regulation of axonogenesis
positive regulation of synapse assembly
cell-cell adhesion
positive regulation of potassium ion transmembrane transport
