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Invitrogen
AMPK alpha-1 Monoclonal Antibody (9Q34)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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AMPK alpha-1 Antibody (AHO1332) in ICC/IF
Immunofluorescence analysis of AMPK alpha-1 was performed using 70% confluent log phase LNCap cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with AMPK alpha-1 Monoclonal Antibody (9Q34)(Product # AHO1332) at 1:100 dilution in 0.1% BSA and incubated overni... View More
产品信息
AHO1332
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
9Q34
抗原
Recombinant fragment of human AMPK-alpha-1 expressed in E. coli.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
purified
保存液
PBS, pH 7.2, with 1% BSA
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536333
靶标信息
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; activated protein kinase; AMP -activate kinase alpha 1 subunit; AMP activated protein kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha-1 chain; AMPK subunit alpha-1; AMPK subunit pAMPK; AMPK-alpha-2 chain; AMPK2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; MGC33776; MGC57364; pAMPK; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 1 catalytic subunit; Tau-protein kinase PRKAA1
基因别名: AI194361; AI450832; AL024255; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; PRKAA1
UniProt ID: (Human) Q13131, (Rat) P54645, (Mouse) Q5EG47
Entrez Gene ID: (Human) 5562, (Rat) 65248, (Mouse) 105787
chromatin binding
protein kinase activity
AMP-activated protein kinase activity
cAMP-dependent protein kinase activity
protein binding
ATP binding
protein C-terminus binding
kinase binding
histone serine kinase activity
metal ion binding
[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity
tau-protein kinase activity
[acetyl-CoA carboxylase] kinase activity
protein serine/threonine kinase activity
eukaryotic elongation factor-2 kinase activator activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
activation of MAPK activity
response to hypoxia
glucose metabolic process
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
fatty acid biosynthetic process
cholesterol biosynthetic process
cell cycle arrest
signal transduction
positive regulation of cell proliferation
lipid biosynthetic process
response to UV
cold acclimation
response to gamma radiation
positive regulation of autophagy
positive regulation of gene expression
response to activity
Wnt signaling pathway
macroautophagy
fatty acid oxidation
response to caffeine
cellular response to nutrient levels
negative regulation of TOR signaling
regulation of peptidyl-serine phosphorylation
histone-serine phosphorylation
intracellular signal transduction
cellular response to glucose starvation
glucose homeostasis
regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of cholesterol biosynthetic process
positive regulation of glycolytic process
negative regulation of glucosylceramide biosynthetic process
rhythmic process
positive regulation of skeletal muscle tissue development
negative regulation of lipid catabolic process
protein heterooligomerization
fatty acid homeostasis
regulation of vesicle-mediated transport
cellular response to hydrogen peroxide
cellular response to ethanol
cellular response to prostaglandin E stimulus
cellular response to organonitrogen compound
cellular response to hypoxia
response to camptothecin
regulation of signal transduction by p53 class mediator
regulation of energy homeostasis
negative regulation of glucose import in response to insulin stimulus
autophagy
negative regulation of translation
response to hydrogen peroxide
positive regulation of gluconeogenesis
positive regulation of fatty acid oxidation
positive regulation of glucose import
lipid metabolic process
fatty acid metabolic process
steroid biosynthetic process
steroid metabolic process
cholesterol metabolic process
sterol biosynthetic process
phosphorylation
chromatin modification
