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AMPK beta-1 Antibody (PA5-114848) in ICC/IF
Immunocytochemistry analysis of AMPK beta-1 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AMPK beta-1 polyclonal antibody (Product # PA5-114848) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjuga... View More
产品信息
PA5-114848
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PRKAB1(Accession Q9Y478), corresponding to amino acid residues K156-L206.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2899484
产品详细信息
Antibody detects endogenous levels of total AMPK beta 1.
靶标信息
AMPK beta 1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5'-AMP-activated protein kinase 40 kDa subunit; 5'-AMP-activated protein kinase beta-1 subunit; 5'-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase, beta subunit; 5-AMP-activated protein kinase beta subunit; AAKB1; AMP-activated protein kinase beta subunit; AMPK beta -1 chain; AMPK beta 1; AMPK beta-1 chain; AMPK subunit beta-1; AMPK-beta1; AMPKb; kinase AMPK-beta1; kinase pAMPK; pAMPK; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1
基因别名: 1300015D22Rik; AMPK; AU021155; E430008F22; HAMPKb; PRKAB1
UniProt ID: (Human) Q9Y478, (Mouse) Q9R078, (Rat) P80386
Entrez Gene ID: (Human) 5564, (Mouse) 19079, (Rat) 83803
protein phosphorylation
fatty acid biosynthetic process
cell cycle arrest
signal transduction
positive regulation of gene expression
macroautophagy
regulation of protein kinase activity
protein heterooligomerization
regulation of signal transduction by p53 class mediator
lipid metabolic process
fatty acid metabolic process
regulation of catalytic activity
