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APAF1 Polyclonal Antibody

查看其他16个APAF1抗体

APAF1 Antibody in Western Blot (WB)

APAF1 Antibody in Western Blot (WB)

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APAF1 Antibody (29022-1-AP150UL) in WB

COLO 320 cells were subjected to SDS PAGE followed by western blot with (Product # 29022-1-AP150UL) (APAF1 antibody) at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

APAF1 Antibody in Western Blot (WB)

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APAF1 Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with (Product # 29022-1-AP150UL) (APAF1 antibody) at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

产品信息

29022-1-AP150UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

靶标信息

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: APAF-1; Apoptotic protease-activating factor 1; DKFZp781B1145

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基因别名: APAF-1; APAF1; CED4; KIAA0413

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UniProt ID: (Human) O14727

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Entrez Gene ID: (Human) 317

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nucleotide binding protein binding ATP binding cysteine-type endopeptidase activator activity involved in apoptotic process heat shock protein binding identical protein binding ADP binding

参与通路

response to hypoxia neural tube closure apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process nervous system development aging response to nutrient activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cardiac muscle cell apoptotic process cell differentiation forebrain development glial cell apoptotic process regulation of apoptotic process positive regulation of apoptotic process protein homooligomerization neuron apoptotic process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to transforming growth factor beta stimulus response to G1 DNA damage checkpoint signaling intrinsic apoptotic signaling pathway regulation of apoptotic DNA fragmentation positive regulation of apoptotic signaling pathway