Invitrogen

APAF1 Polyclonal Antibody

APAF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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APAF1 Antibody (PA5-85121) in IHC (P)

APAF1 Polyclonal Antibody detects APAF1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. APAF1 Polyclonal Antibody (Product # PA5-85121) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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产品信息

PA5-85121

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the C-terminus region of human APAF1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.51 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2792268

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (85%), Rat (85%), Bovine (90%).

Positive Control: Target recombinant protein.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pulmonary Exposure to Magnéli Phase Titanium Suboxides Results in Significant Macrophage Abnormalities and Decreased Lung Function.

Frontiers in immunology

McDaniel DK,Ringel-Scaia VM,Morrison HA,Coutermarsh-Ott S,Council-Troche M,Angle JW,Perry JB,Davis G,Leng W,Minarchick V,Yang Y,Chen B,Reece SW,Brown DA,Cecere TE,Brown JM,Gowdy KM,Hochella MF,Allen IC

PA5-85121 was used in Immunohistochemistry on paraffin embedded tissues to determine the clinical and physiological impact of pulmonary exposure to Magneli phases using mice as mammalian model organisms.