Invitrogen

APAF1 Monoclonal Antibody (2E10)

查看其他16个APAF1抗体

APAF1 Antibody in Immunocytochemistry (ICC/IF)

APAF1 Antibody in Immunocytochemistry (ICC/IF)

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APAF1 Antibody (MA5-15743) in ICC/IF

Immunocytochemistry analysis of Apaf1 in K562 cells with APAF1 Monoclonal Antibody (2E10) (Product # MA5-15743) at a dilution of 0.5 µg/mL.

APAF1 Antibody in Immunocytochemistry (ICC/IF)

APAF1 Antibody in Western Blot (WB)

Product Image Gallery

APAF1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Apaf1 in K562 cells with APAF1 Monoclonal Antibody (2E10) (Product # MA5-15743) at a dilution of 0.5 µg/mL.

APAF1 Antibody in Western Blot (WB) — Western Blot analysis of Apaf1 in K562 cell lysate with APAF1 Monoclonal Antibody (2E10) (Product # MA5-15743) at (A) 0.25 and (B) 0.5 mg/mL.

产品信息

MA5-15743

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

-

免疫组化 (IHC)

0.5 µg/mL

-

免疫细胞化学 (ICC/IF)

0.5 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2E10

抗原

A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11154527

产品详细信息

In Western blot applications, this antibody detects a band at ~130kDa. A suggested positive control is K562 cell lysate.

靶标信息

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: APAF-1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; DKFZp781B1145; forebrain overgrowth

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基因别名: 6230400I06Rik; APAF-1; APAF1; Apaf1l; CED4; fog; KIAA0413; mKIAA0413

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UniProt ID: (Human) O14727, (Rat) Q9EPV5, (Mouse) O88879

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Entrez Gene ID: (Human) 317, (Rat) 78963, (Mouse) 11783

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nucleotide binding protein binding ATP binding cysteine-type endopeptidase activator activity involved in apoptotic process heat shock protein binding identical protein binding ADP binding

参与通路

response to hypoxia neural tube closure apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process multicellular organism development brain development aging response to nutrient activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cardiac muscle cell apoptotic process cell differentiation forebrain development glial cell apoptotic process protein homooligomerization neuron apoptotic process cellular response to transforming growth factor beta stimulus apoptotic signaling pathway regulation of apoptotic DNA fragmentation positive regulation of apoptotic signaling pathway nervous system development regulation of apoptotic process positive regulation of apoptotic process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress response to G1 DNA damage checkpoint signaling intrinsic apoptotic signaling pathway