Invitrogen

ARF5 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

查看其他8个ARF5抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

ARF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ARF5 Antibody (PA5-31432) in IHC (P)

Immunohistochemistry (Paraffin) analysis of ARF5 was performed in paraffin-embedded mouse lung tissue using ARF5 Polyclonal Antibody (Product # PA5-31432) at a dilution of 1:500.

Product Image Gallery

ARF5 Antibody — Fig 6 Arf6 is recruited by R. typhi RalF and is required for infection. (A) Ectopically expressed RalFRtFL co-localizes with Arf6 but not Arf5. HeLa cells co-expressing EYFP, EYFP-RalFRtFL or EYFP-RalFRbFL and mRFP-Arf6 (left) or -Arf5 (right) were fixed with 4% para-formaldehyde. Nuclei were stained with DAPI (blue). (Scale bar: 10 µm). (B) RalFRtFL pull-down of Arf6. Lysates from HEK293T cells expressing mRFP-Arf5 or -Arf6 were incubated with HisPur Cobalt resin bound with rHis-RalFRtFL or resin alone. Bound proteins were eluted with imidazole and analyzed by protein immunoblot using antibodies as indicated. (C) Arf6 is recruited during R. typhi entry. HeLa cells expressing mRFP-Arf5 or -Arf6 (red) were infected with partially purified R. typhi (MOI ~100). Ten minutes post infection, cells were fixed and R. typhi detected with anti-R. typhi serum (green). DAPI (blue) is shown in the merged image. Boxed regions are enlarged to show detail. White arrowheads indicate R. typhi. (Scale bar: 5 µm). (D) Arf6 knockdown inhibits R. typhi infection. HeLa cells transfected with negative, Arf6, or Arf5 siRNA were infected with partially purified R. typhi (MOI ~100). At 2 hrs post infection, cells were fixed, plasma membrane stained with Alexa Fluor 594 wheat germ agglutinin, and R. typhi detected with rat anti-R. typhi serum and Alexa Fluor 488 anti-rat antibody. The number of R. typhi per host cell was counted for 100 host cells for three independent experiments. Error bars represent mean ± SD (Student’s two-sided t-test). (Scale bar: 5µm). (E) Confirmat {KD}

产品信息

PA5-31432

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 161 of Human ARF5

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.43 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2548906

产品详细信息

Recommended positive controls: 293T, Raji, mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (85%), Pig (100%), Chicken (98%), Sheep (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。