Invitrogen

ASH2L Polyclonal Antibody

ASH2L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ASH2L Antibody (PA5-119994) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast carcinoma tissue using ASH2L Polyclonal Antibody (Product # PA5-119994). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ASH2L antibody at a d... View More

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产品信息

PA5-119994

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within human ASH2L aa 575-628

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2913566

产品详细信息

Positive Control: Hela cell lysates, PC-12 cell lysates, mouse colon tissue, human breast carcinoma tissue, human colon tissue, Siha.

Subcellular Location: Nucleus.

靶标信息

The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Its predicted molecular weight is 68.7kDa. Alternative splicing results in possible isoforms at 60 kDa & 56.5 kDa). Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. Homology to mouse ASH2L is 90%. ASH2L is predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes as well as leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cell lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. In addition ASH2L has been suggested to interact with beta-tubulin and beta-catenin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ASH 2; ash2 (absent, small, or homeotic)-like; ASH2 LIKE; ASH2 like protein; ASH2-like protein; Bre 2; Set1/Ash2 histone methyltransferase complex subunit ASH2

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基因别名: ASH2; ASH2L; ASH2L1; ASH2L2; Bre2

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UniProt ID: (Human) Q9UBL3, (Mouse) Q91X20

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Entrez Gene ID: (Human) 9070, (Mouse) 23808, (Rat) 290829

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功能

DNA binding protein binding beta-catenin binding histone-lysine N-methyltransferase activity histone methyltransferase activity (H3-K4 specific) transcription regulatory region DNA binding metal ion binding euchromatin binding chromatin/chromatin-binding, or -regulatory protein histone modifying enzyme

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