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ATF6 Antibody (A303-720A) in IHC
Detection of human ATF6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- ATF6 (Cat. No. A303-720A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).
产品信息
A303-720A
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 620 and 670 of human Activating Transcription Factor 6.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
phosphate/tris citrate, pH 7-8
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
靶标信息
ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activating transcription factor 6 alpha; Activating transcription factor 6 beta; ATF 6; ATF6 alpha; ATF6-alpha; ATF6-beta; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; cAMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 beta; cAMP-responsive element-binding protein-like 1; Creb-related protein; Creb-rp; Cyclic AMP-dependent transcription factor ATF-6 alpha; Cyclic AMP-dependent transcription factor ATF-6 beta; DKFZp686P2194; FLJ21663; Protein G13
基因别名: ACHM7; ATF6; ATF6A; ATF6B; CREB-RP; CREBL1; G13
UniProt ID: (Human) P18850, (Human) Q99941
Entrez Gene ID: (Human) 22926, (Human) 1388
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
ubiquitin protein ligase binding
cAMP response element binding
identical protein binding
protein heterodimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
eye development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein folding
response to stress
positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response
signal transduction
visual perception
endoplasmic reticulum unfolded protein response
ATF6-mediated unfolded protein response
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
