Invitrogen

ATF6 Polyclonal Antibody

ATF6 Antibody in Immunocytochemistry (ICC/IF)

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ATF6 Antibody (PA5-68556) in ICC/IF

Immunofluorescence analysis of ATF6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ATF6 Polyclonal Antibody (Product # PA5-68556) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

ATF6 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-68556

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1.0 µg/mL

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免疫组化 (IHC)

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

synthetic peptide corresponding to a region of mouse ATF6

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2688633

产品详细信息

This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 85%; Rabbit: 100%; Rat: 92%

靶标信息

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

PA5-68556 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 EST 2020

生物信息学

蛋白别名: Activating transcription factor 6 alpha; ATF 6; ATF6 alpha; ATF6-alpha; ATF6-beta; cAMP response element binding protein-related protein; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; cAMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 beta; cAMP-responsive element-binding protein-like 1; Cyclic AMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 beta; DKFZp686P2194; FLJ21663

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基因别名: 9130025P16Rik; 9630036G24; AA617266; AA789574; Atf6; Atf6alpha; ATF6beta; Creb-rp; Crebl1; ESTM49

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UniProt ID: (Mouse) F6VAN0

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Entrez Gene ID: (Mouse) 226641, (Mouse) 12915

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功能

RNA polymerase II regulatory region sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding cAMP response element binding sequence-specific DNA binding protein heterodimerization activity transcription regulatory region DNA binding

参与通路

transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter response to unfolded protein endoplasmic reticulum unfolded protein response positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of transcription from RNA polymerase II promoter