Invitrogen

ATG12-ATG5 Recombinant Rabbit Monoclonal Antibody (20H20L20)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

说明书

实验方案

技术支持

说明书

实验方案

技术支持

ATG12-ATG5 Antibody in Western Blot (WB)

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ATG12-ATG5 Antibody (702433) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK-293 (Lane1), THP-1 (Lane2), PANC-1 (Lane3), HCT 116 (Lane4), Mouse Colon (Lane5) and Mouse Pancreas (Lane6). The blots were probed with ATG12-ATG5 Recombinant Rabbit Monoclonal Antibody (Product # 702433, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to the covalently conjugated ATG12-ATG5 and 17 kDa... View More

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产品信息

702433

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

20H20L20

抗原

Peptides corresponding to Human ATG12 (aa 43-57, 122-133)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2633090

产品详细信息

This antibody is predicted to react with Monkey, Pig, Cat and Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Apg12 (autophagy, yeast) homolog; APG12-like; APG5-like; Apoptosis-specific protein; ATG12 autophagy related 12 homolog; ATG5 autophagy related 5 homolog; Autophagy protein 5; autophagy-related 12; autophagy-related 5; Autophagy-related protein 12; Ubiquitin-like protein ATG12

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基因别名: 2010107M05Rik; 3110067M24Rik; 4931423H11Rik; A330058M13Rik; APG12; APG12L; APG5; APG5-LIKE; APG5L; ASP; ATG12; Atg12l; ATG5; Atg5l; AW319544; C88337; FBR93; HAPG12; hAPG5; Paddy