Invitrogen

ATG12 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

查看其他18个ATG12抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (5)
高级验证 (1)

ATG12 Antibody in Immunocytochemistry (ICC/IF)

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ATG12 Antibody (PA5-17218) in ICC/IF

Immunofluorescence analysis of ATG12 was performed using 70% confluent log phase NIH/3T3 cells treated with 50uM of Rapamycin for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ATG12 Polyclonal Antibody (Product # PA5-17218) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More

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ATG12 Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using ATG12 Polyclonal Antibody (Product # PA5-17218), shows increased expression of protein in NIH/3T3 cell line upon treatment with Rapamycin. {TM}

产品信息

PA5-17218

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:50

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues near the amino terminus of mouse Atg12

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

29 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10985246

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。