Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 9
ATG5 Antibody (PA1-46178) in ICC/IF
Immunofluorescence analysis of ATG5 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATG5 Rabbit Polyclonal Antibody (Product # PA1-46178) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
产品信息
PA1-46178
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Fruit fly,
Fish,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Reptile,
Rat,
Xenopus,
Zebrafish
已发表种属
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide made to an N-terminal region of the human ATG5 protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2274520
产品详细信息
In Western Blot, a band is seen ~56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is ~33 kDa.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: antigen standard for ATG5 (transient overexpression lysate), mouse wildtype ES cell lysate.
靶标信息
ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: APG 5; APG5 autophagy 5 like (APG5L); APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 homolog; Atg5-PA; autophagy 5-like protein; Autophagy protein 5; autophagy related 5-like protein; autophagy-related 5; autophagy-specific gene 5; CG1643-PA; LD34980p
基因别名: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; Atg-5; ATG5; Atg5l; AW319544; C88337; CG1643; DmAtg5; Dmel\CG1643; Dmel_CG1643; hAPG5; Paddy; XELAEV_18027227mg; zgc:100934
UniProt ID: (Pig) Q3MQ04, (Human) Q9H1Y0, (Bovine) Q3MQ24, (Fruit fly) Q9W3R7, (Mouse) Q99J83
immune system process
autophagy
apoptotic process
regulation of cilium assembly
autophagosome assembly
mitophagy
blood vessel remodeling
regulation of cytokine secretion involved in immune response
C-terminal protein lipidation
cellular response to nitrogen starvation
response to fungus
macroautophagy
cellular homeostasis
antigen processing and presentation of endogenous antigen
negative regulation of protein ubiquitination
aggrephagy
negative stranded viral RNA replication
vasodilation
response to drug
negative regulation of apoptotic process
post-translational protein modification
nucleophagy
negative thymic T cell selection
innate immune response
otolith development
regulation of release of sequestered calcium ion into cytosol
ventricular cardiac muscle cell development
heart contraction
negative regulation of cell death
protein lipidation involved in autophagosome assembly
positive regulation of mucus secretion
positive regulation of mitophagy
negative regulation of reactive oxygen species metabolic process
negative regulation of histone H4-K16 acetylation
negative regulation of phagocytosis
cellular response to nitrosative stress
autophagy of host cells involved in interaction with symbiont
cellular response to starvation
larval midgut cell programmed cell death
glycophagy
regulation of reactive oxygen species metabolic process
nervous system development
heart development
regulation of skeletal muscle tissue regeneration
