3万+试剂耗材飞享价超值购！2025年首单即送500积分，订购多款产品可享额外积分奖励！

进入促销商城

Search Thermo Fisher Scientific

联系我们

货号直购

登录
登录

没有账号？建立账号注册

Loading ...

Invitrogen

ATGL Monoclonal Antibody (K.751.7)

9个已发表图片

6 篇参考文献

查看其他16个ATGL抗体

问题与答案

货号 MA5-14990

Price (CNY)

4,317.00

100 µL

下单后预计有货时间 23-Apr-2025

批量或定制请求

问题与答案

Application

ATGL Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 16

ATGL Antibody (MA5-14990) in ICC/IF

Immunofluorescence analysis of ATGL was performed using 70% confluent log phase 3T3-L1 and 3T3-L1 differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Anti... View More

ATGL Antibody in Immunocytochemistry (ICC/IF)

ATGL Antibody in Immunocytochemistry (ICC/IF)

ATGL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot (WB)

ATGL Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

ATGL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ATGL was performed using 70% confluent log phase 3T3-L1 and 3T3-L1 differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to vacuolar structure (fat droplets) in cytoplasm. Panel e shows 3T3-L1 cells with no expression of ATGL. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ATGL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ATGL in 3T3-L1 cells using an ATGL monoclonal antibody (Product # MA5-14990) (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.

ATGL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ATGL in paraffin-embedded mouse brown fat using an ATGL monoclonal antibody (Product # MA5-14990).

ATGL Antibody in Western Blot (WB) — Western blot was performed using ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) and a 55 kDa band corresponding to ATGL was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Raw 264.7 (Lane 1), Mouse adipose (Lane 2), Mouse skeletal muscle (Lane 3), Rat adipose (Lane 4), 3T3-L1 (Lane 5), 3T3-L1 differentiated to adipocytes (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

ATGL Antibody in Western Blot (WB) — Western blot analysis of ATGL in extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells using ATGL monoclonal antibody (Product # MA5-14990).

ATGL Antibody in Western Blot (WB) — Ca 2+ oscillations induced by Ca 2+ -free medium application activate the lipolysis process and correlate with changes in the expression of key genes in white adipose tissue. ( A , B ) Control ( A ) white adipocytes and cells with Cx43 gene knockdown using Gja1 siRNA (Cx43-KD, ( B )) were exposed to Ca 2+ -free medium for 1 h and then returned to a CO 2 incubator for 24 h. Red line--Ca 2+ oscillations in 29 +- 14% and Ca 2+ transients (blue curve) in the 47 +- 9% of white adipocytes. In Panel ( B )--black curve (+CBX): Ca 2+ -free medium application was performed with 100 uM CBX. ( C ) Effects of the Ca 2+ -free medium on the expression of genes in white adipocytes + CBX and Cx43 gene knockdown cells (Cx43-KD) after 24 h. All values are given as the mean +- SEM. Scrambled--cultures transfected with an siRNA whose sequence differed from that of the siRNA against Cx43. Gene expression level was normalized to the reference gene Gapdh and was presented relative to the control (adipocytes were not exposed to Ca 2+ -free medium), which was considered as 1 (dashed line). A comparison of the experimental groups with the control was made. The differences between the experimental groups and control are marked with asterisks: * p < 0.05, ** p < 0.01, and *** p < 0.001. n/s--no significant difference. ( D ) The protein abundance of LIPE and ATGL in control, Cx43-KD, CBX-treated, Ca 2+ -free-treated, CBX + Ca 2+ -free-treated and CX43-KD + Ca 2+ -free-treated white adipocytes. The data we

ATGL Antibody in Western Blot (WB) — Figure 5 Effects of MJ2 and hkMJ2 on protein expression levels related to adipogenesis and lipid metabolism in eWAT. Total protein was extracted from eWAT in each group, and the relative protein expression levels of the factors related to ( A ) adipogenesis (PPARgamma and C/EBPalpha), ( B ) lipogenesis (FAS, SCD-1 and ACC) and ( C ) lipolysis (ATGL and HSL) were investigated by western blot. Representative images of each protein are shown, and the relative quantified expression levels indicate the mean +- SD. The p values were determined by ANOVA and Tukey's HSD test. The full-length blots are shown in Supplementary Fig. 3 .

ATGL Antibody in Western Blot (WB) — Figure 4 Cardiac-specific ATGL overexpression prevents lipid accumulation following burn injury. (a) Identification of WT and MHC-ATGL Het mice by PCR analysis. (b, c) Immunoblot analysis showing ATGL expression in WT and MHC-ATGL Het mice under basal conditions; n = 6. (d, e) Analysis comparing serum TG and FFA between WT and MHC-ATGL Het mice with or without burn injury; n = 10 - 35. (f, g) Cardiac TG and FFA were compared between WT and MHC-ATGL Het mice with or without burn stimulation; n = 13 - 37. MHC-ATGL denotes MHC-ATGL Het. Data are shown as the mean +- SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001. P values were derived from two-tailed Student's t -test (c) and one-way ANOVA with the appropriate posttest (d-g).

ATGL Antibody in Western Blot (WB) — Figure 6 Role of ATGL in fat and glucose metabolisms. (a) ATP content of the heart was detected in ATGL-Het, MHC-ATGL Het, and WT mice before or after burn challenge; n = 15 - 34. (b, c) Palmitate oxidation and glucose oxidation were compared between the different genotypes of mice with or without burn challenge; n = 8. (d-g) Protein expression of ATGL, CD36, PPAR alpha , PGC1 alpha , GLUT1, and GLUT4 in WT, ATGL-Het, and MHC-ATGL Het mice was detected by immunoblot analysis with or without burn challenge; n = 6. Data are shown as the mean +- SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001. P values were derived from one-way ANOVA with the appropriate posttest.

ATGL Antibody in Western Blot (WB) — Figure 1 Increased ATGL expression in the heart of burned mice. (a-d) Hematoxylin-eosin staining of skin histological sections with or without burn injury. Panels (b, d) showed high-magnification image of the inset in panels (a, c). Bar: 50 mu m (a, c) or 20 mu m (b, d). (e) Cardiac ATGL mRNA was detected by reverse transcription-polymerase chain reaction with or without burn injury; n = 6. (f, g) Cardiac ATGL protein level was examined by immunoblot analysis; n = 6. Data are shown as the mean +- SEM. ** P < 0.01 and *** P < 0.001. P values were derived from two-tailed Student's t -test.

ATGL Antibody in Western Blot (WB) — Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

ATGL Antibody in Western Blot (WB) — Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

ATGL Antibody in Western Blot (WB) — Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

ATGL Antibody in Western Blot, Immunoprecipitation (WB, IP) — Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot and Immunoprecipitation

ATGL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) shows increased expression of ATGL in 3T3-L1 differentiated adipocytes in comparison to 3T3-L1. {RE}

ATGL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Higher expression of ATGL was observed in 3T3-L1 differentiated adipocytes in comparison to 3T3-L1 using ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) in western blot. {RE}

产品信息

MA5-14990

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50

-

免疫细胞化学 (ICC/IF)

1:100

-

免疫沉淀 (IP)

1:50

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

K.751.7

抗原

Synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

23 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10974333

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Thu Jan 28 00:00:00 UTC 2021

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Thu Jan 28 00:00:00 UTC 2021

Activation of Cx43 Hemichannels Induces the Generation of Ca²⁺ Oscillations in White Adipocytes and Stimulates Lipolysis.

International journal of molecular sciences

Turovsky EA,Varlamova EG,Turovskaya MV

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Wed Jul 28 00:00:00 UTC 2021

Activation of Cx43 Hemichannels Induces the Generation of Ca²⁺ Oscillations in White Adipocytes and Stimulates Lipolysis.

International journal of molecular sciences

Turovsky EA,Varlamova EG,Turovskaya MV

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Wed Jul 28 00:00:00 UTC 2021

Myocardial Adipose Triglyceride Lipase Overexpression Protects against Burn-Induced Cardiac Lipid Accumulation and Injury.

Oxidative medicine and cellular longevity

Li L,Zhang X,Zhang Q,Jia J,Zhang J,Zhang D,Song H,Chen B,Hu J,Huang Y

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Fri Dec 13 00:00:00 UTC 2019

Atgl deficiency induces podocyte apoptosis and leads to glomerular filtration barrier damage.

The FEBS journal

Chen W,Jiang Y,Han J,Hu J,He T,Yan T,Huang N,Zhang Q,Mei H,Liao Y,Huang Y,Chen B

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Sat Apr 01 00:00:00 UTC 2017

Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.

The Journal of biological chemistry

Grahn THM,Kaur R,Yin J,Schweiger M,Sharma VM,Lee MJ,Ido Y,Smas CM,Zechner R,Lass A,Puri V

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Fri Apr 25 00:00:00 UTC 2014

Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.

The Journal of biological chemistry

Grahn THM,Kaur R,Yin J,Schweiger M,Sharma VM,Lee MJ,Ido Y,Smas CM,Zechner R,Lass A,Puri V

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Fri Apr 25 00:00:00 UTC 2014

生物信息学

蛋白别名: Adipose triglyceride lipase; ATGL DESNUTRIN; Calcium-independent phospholipase A2-zeta; Desnutrin; DKFZp667M109; IPLA2 zeta; iPLA2-zeta; Patatin-like phospholipase domain-containing protein 2; PEDF R; plpl; plpl2; transport-secretion protein; triglyceride hydrolase; TTS 2.2

基因别名: 0610039C21Rik; 1110001C14Rik; Atgl; Pnpla2; RGD1309044; TTS-2.2

UniProt ID: (Rat) P0C548, (Mouse) Q8BJ56

Entrez Gene ID: (Rat) 361676, (Mouse) 66853

功能

参与通路

negative regulation of sequestering of triglyceride positive regulation of triglyceride catabolic process triglyceride catabolic process lipid metabolic process metabolic process lipid catabolic process lipid storage lipid particle organization

1x1 image pixel for data collection