Invitrogen

ATP Citrate Lyase Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他31个ATP Citrate Lyase抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (13)
高级验证 (1)

ATP Citrate Lyase Antibody in Immunocytochemistry (ICC/IF)

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ATP Citrate Lyase Antibody (PA5-78712) in ICC/IF

Immunocytochemistry analysis of ACLY using anti-ACLY antibody (Product # PA5-78712) . ACLY was detected in an immunocytochemical section of A594 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-ACLY antibody (Product # PA5-78712) overnight at 4°C. DyLight®488 Conjugated... View More

ATP Citrate Lyase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP Citrate Lyase Antibody in Western Blot (WB)

ATP Citrate Lyase Antibody in Flow Cytometry (Flow)

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产品信息

PA5-78712

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.25 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2745828

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

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