Invitrogen

ATP Citrate Lyase Polyclonal Antibody

ATP Citrate Lyase Antibody in Western Blot (WB)

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ATP Citrate Lyase Antibody (PA5-80298) in WB

Western Blot using ATP Citrate Lyase Polyclonal Antibody (Product # PA5-80298) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 120 kDa. Observed band size: 120 kDa. (We are unsure of the identity of these extra bands).

ATP Citrate Lyase Antibody in Immunoprecipitation (IP)

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产品信息

PA5-80298

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1:5,000-1:10,000

免疫沉淀 (IP)

1-4 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human ACLY/ATP Protein (Met1-Met1101)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2787629

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human ACLY/ATP.

靶标信息

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。