Invitrogen

ATP Synthase beta Monoclonal Antibody (4.3E8.D10), Alexa Fluor™ 555

ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)

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ATP Synthase beta Antibody (MA1-930-A555) in ICC/IF

Immunofluorescent analysis of ATP Synthase Beta (orange) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ATP Synthase beta mouse monoclonal antibody, AlexaFluor 555 conjugate (Product # MA1-930-A555), at a concentration of 5 µg/mL in blocking buffer... View More

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产品信息

MA1-930-A555

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1-10 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

4.3E8.D10

抗原

Intact rat mitochondria

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偶联物

Alexa Fluor™ 555 Alexa Fluor™ 555 Alexa Fluor™ 555

激发/发射光谱

553/568 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2633316

产品详细信息

MA1930A555 detects the beta subunit of ATP synthase from mouse rat and human samples. This antibody is useful as a mitochondrial marker.

靶标信息

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hexokinase 1 cellular localization regulates the metabolic fate of glucose.

Molecular cell

De Jesus A,Keyhani-Nejad F,Pusec CM,Goodman L,Geier JA,Stoolman JS,Stanczyk PJ,Nguyen T,Xu K,Suresh KV,Chen Y,Rodriguez AE,Shapiro JS,Chang HC,Chen C,Shah KP,Ben-Sahra I,Layden BT,Chandel NS,Weinberg SE,Ardehali H

MA1-930-A555 was used in Immunocytochemistry to indicate that HK1 mitochondrial binding alters glucose metabolism through regulation of GAPDH.

Thu Apr 07 00:00:00 EDT 2022

生物信息学

蛋白别名: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

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基因别名: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271

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UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480

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Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947

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