Invitrogen

ATR Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

20 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 8

ATR Antibody (PA1-450) in WB

Western blot analysis of ATR was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against ATR (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 ... View More

ATR Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

ATR Antibody — The specificity of anti-ATR polyclonal antibody (Product # PA1-450) was demonstrated by siRNA knockdown. Western blot analysis of HeLa cell extracts using this antibody showed decreased level of ATR protein expression in cells transfected with ATR specific siRNA (lane 3) but not in controls (lane 1 and 2). {KD}

产品信息

PA1-450

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看17篇已发表文章 17篇已发表文章

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Fusion protein containing residues 400-460 of the human ATR protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

4 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325822

产品详细信息

PA1-450 detects ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein) from human cells.

PA1-450 has been successfully used in Western blot immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~305 kDa protein representing ATR in lysate from UV irradiated K562 cells.

PA1-450 antigen is a fusion protein corresponding to amino acid residues 400-460 from human ATR.

靶标信息

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Loss of MAP3K7 Sensitizes Prostate Cancer Cells to CDK1/2 Inhibition and DNA Damage by Disrupting Homologous Recombination.

Molecular cancer research : MCR

Washino S,Rider LC,Romero L,Jillson LK,Affandi T,Ohm AM,Lam ET,Reyland ME,Costello JC,Cramer SD

PA1-450 was used in Western Blotting to demonstrate that loss of MAP3K7 is a main contributing factor to drug response through disruption of HR in prostate cancer.

Tue Oct 01 00:00:00 EDT 2019

The Ataxia telangiectasia-mutated and Rad3-related protein kinase regulates cellular hydrogen sulfide concentrations.

DNA repair

Chen J,Shen X,Pardue S,Meram AT,Rajendran S,Ghali GE,Kevil CG,Shackelford RE

Published figure using ATR polyclonal antibody (Product # PA1-450) in Western Blot

Tue Jan 01 00:00:00 EST 2019

Atrazine Triggers DNA Damage Response and Induces DNA Double-Strand Breaks in MCF-10A Cells.

International journal of molecular sciences

Huang P,Yang J,Ning J,Wang M,Song Q

Published figure using ATR polyclonal antibody (Product # PA1-450) in Western Blot

Wed Jun 24 00:00:00 EDT 2015

Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1.

Molecular biology of the cell

Meng Z,Capalbo L,Glover DM,Dunphy WG

PA1-450 was used in western blot to study the role of casein kinase 1 in checkpoint responses to stalled DNA replication forks

Mon Aug 15 00:00:00 EDT 2011

ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function.

Blood

Collins NB,Wilson JB,Bush T,Thomashevski A,Roberts KJ,Jones NJ,Kupfer GM

PA1-450 was used in immunoprecipitation to study the role of FANCA phosphorylation in Fanconi anemia.

Thu Mar 05 00:00:00 EST 2009

ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function.

Blood

Collins NB,Wilson JB,Bush T,Thomashevski A,Roberts KJ,Jones NJ,Kupfer GM

PA1-450 was used in immunoprecipitation to study the role of FANCA phosphorylation in Fanconi anemia.

Thu Mar 05 00:00:00 EST 2009

DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.

Cell death and differentiation

Carlessi L,De Filippis L,Lecis D,Vescovi A,Delia D

PA1-450 was used in western blot to evaluate an immortalized human neural stem-cell line as an in vitro model for the study of neuronal DNA-damage response

Mon Jun 01 00:00:00 EDT 2009

BRCA1 activates a G2-M cell cycle checkpoint following 6-thioguanine-induced DNA mismatch damage.

Cancer research

Yamane K,Schupp JE,Kinsella TJ

PA1-450 was used in western blot to investigate the role of BRCA1 in the G (2)-M checkpoint response to 6-thioguanine-induced DNA mismatch damage

Sun Jul 01 00:00:00 EDT 2007

The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres.

Cell

Verdun RE,Karlseder J

PA1-450 was used in western blot to investigate the involvement of DNA damage machinery and homologous recombination pathway in the maintenance of telomere integrity

Fri Nov 17 00:00:00 EST 2006

ATR functions as a gene dosage-dependent tumor suppressor on a mismatch repair-deficient background.

The EMBO journal

Fang Y,Tsao CC,Goodman BK,Furumai R,Tirado CA,Abraham RT,Wang XF

PA1-450 was used in immunoprecipitation to study the interaction between MMR proteins and ATR during the cellular response to genotoxic stress.

Wed Aug 04 00:00:00 EDT 2004

Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling.

The EMBO journal

Tsao CC,Geisen C,Abraham RT

PA1-450 was used in western blot to study the role of hMCM7 in the DNA damage signal transmission of from active replication forks to the S-phase checkpoint.

Wed Nov 24 00:00:00 EST 2004

The ATR-p53 pathway is suppressed in noncycling normal and malignant lymphocytes.

Oncogene

Jones GG,Reaper PM,Pettitt AR,Sherrington PD

PA1-450 was used in western blot to study the ATR-p53 pathway in normal and malignant lymphocytes

Thu Mar 11 00:00:00 EST 2004

Quaternary structure of ATR and effects of ATRIP and replication protein A on its DNA binding and kinase activities.

Molecular and cellular biology

Unsal-Kaçmaz K,Sancar A

PA1-450 was used in western blot to study ATR structure and its assocation with DNA.

Sun Feb 01 00:00:00 EST 2004

Mismatch repair-mediated G2/M arrest by 6-thioguanine involves the ATR-Chk1 pathway.

Biochemical and biophysical research communications

Yamane K,Taylor K,Kinsella TJ

PA1-450 was used in western blot to study the involvement of ATR-Chk1 pathway in the inhibitory effect of 6-thioguanine on DNA mismatch repair

Fri May 21 00:00:00 EDT 2004

DNA replication defects, spontaneous DNA damage, and ATM-dependent checkpoint activation in replication protein A-deficient cells.

The Journal of biological chemistry

Dodson GE,Shi Y,Tibbetts RS

PA1-450 was used in western blot to investigate the role of replication protein A during the DNA replication.

Fri Aug 06 00:00:00 EDT 2004

Determination of the catalytic activities of mTOR and other members of the phosphoinositide-3-kinase-related kinase family.

Methods in molecular biology (Clifton, N.J.)

Chiang GG,Abraham RT

PA1-450 was used in western blot to investigate the function of proteins from phosphoinositide-3-kinase-related family

Thu Sep 16 00:00:00 EDT 2004

ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation.

The Journal of biological chemistry

Kozlov S,Gueven N,Keating K,Ramsay J,Lavin MF

Published figure using ATR polyclonal antibody (Product # PA1-450) in Immunoprecipitation

Fri Mar 14 00:00:00 EST 2003

ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation.

The Journal of biological chemistry

Kozlov S,Gueven N,Keating K,Ramsay J,Lavin MF

Published figure using ATR polyclonal antibody (Product # PA1-450) in Immunoprecipitation

Fri Mar 14 00:00:00 EST 2003

An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage.

Molecular and cellular biology

Heffernan TP,Simpson DA,Frank AR,Heinloth AN,Paules RS,Cordeiro-Stone M,Kaufmann WK

PA1-450 was used in western blot to study the roles of checkpoint proteins on replication initiation after UV irradiation

Sun Dec 01 00:00:00 EST 2002

ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK.

Oncogene

Hall-Jackson CA,Cross DA,Morrice N,Smythe C

PA1-450 was used in western blot to study the caffeine-sensitivity and the relation to DNA-PK of DNA-activated protein kinase ATR

Thu Nov 18 00:00:00 EST 1999

生物信息学

蛋白别名: 2310008J16Rik; 2810405N18Rik; Ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8

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基因别名: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1

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UniProt ID: (Human) Q13535

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Entrez Gene ID: (Human) 545

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功能

DNA binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding MutLalpha complex binding MutSalpha complex binding non-receptor serine/threonine protein kinase protein modifying enzyme

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