Invitrogen

Actin (muscle) Monoclonal Antibody (HHF35), Alexa Fluor™ 488, eBioscience™

Actin (muscle) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

Actin (muscle) Antibody (53-6496-82) in ICC/IF

Immunocytochemistry on fixed and permeabilized C2C12 cells stained with 30 µg/mL Mouse IgG1 Alexa Fluor® 488 Isotype Control (left) or 30 µg/mL Anti-Actin (muscle) Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Actin (muscle) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

53-6496-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

30 µg/mL

产品规格

种属反应

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HHF35

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574433

产品详细信息

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.

The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin.

Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This HHF35 antibody has been tested by immunocytochemistry and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Adult Pgf^-/- mice behaviour and neuroanatomy are altered by neonatal treatment with recombinant placental growth factor.

Scientific reports

Kay VR,Cahill LS,Hanif A,Sled JG,Carmeliet P,Tayade C,Croy BA

53-6496 was used in Immunohistochemistry-wholemount to investigate whether developmentally asynchronous (i.e. neonatal) PGF supplementation alters the vascular, neuroanatomic and/or behavioral status of Pgf-/- mice at adulthood.

Wed Jun 26 00:00:00 EDT 2019

Restoration of Corneal Transparency by Mesenchymal Stem Cells.

Stem cell reports

Mittal SK,Omoto M,Amouzegar A,Sahu A,Rezazadeh A,Katikireddy KR,Shah DI,Sahu SK,Chauhan SK

53-6496 was used in Immunohistochemistry to demonstrate that mesenchymal stem cells can restore corneal transparency by secreting high levels of hepatocyte growth factor.

Tue Oct 11 00:00:00 EDT 2016

生物信息学

蛋白别名: ACTB; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin; alpha actin 1; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; B-actin; beta actin; beta-actin FE-3; cardiac actin; cardiac alpha actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; RP5-1068B5.2; skeletal alpha actin; skeletal muscle alpha-actin 1; smooth muscle alpha-actin; Smooth muscle gamma-actin; vascular smooth muscle alpha-actin

查看更多收起

基因别名: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMGA; VSCM