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Bioss

Aldolase A, Aldolase B Polyclonal Antibody

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)

Application

Aldolase A, Aldolase B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 1

Aldolase A, Aldolase B Antibody (BS-2406R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Aldolase Polyclonal Antibody, Unconjugated (bs-2406R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and... View More

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Product Details

BS-2406R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:20-1:100

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Aldolase A.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aldolase 1; aldolase 1, A isoform; Aldolase 2; aldolase 2, B isoform; aldolase A, fructose-bisphosphate; Aldolase B fructose-biphosphate; Aldolase B, fructose-biphosphate; aldolase B, fructose-bisphosphatase; aldolase B, fructose-bisphosphate; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase A; Fructose-bisphosphate aldolase B; Liver-type aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase

Gene Aliases: ALDA; ALDB; Aldo-1; Aldo-2; Aldo1; ALDO2; ALDOA; ALDOB; BC016435; GSD12; HEL-S-87p; LIV10; RNALDOG5

UniProt ID: (Human) P04075, (Human) P05062, (Mouse) P05064, (Rat) P05065, (Mouse) Q91Y97

Entrez Gene ID: (Human) 226, (Human) 229, (Mouse) 11674, (Rat) 24189, (Mouse) 230163, (Rat) 24190

Function(s)

actin binding fructose-bisphosphate aldolase activity protein binding cytoskeletal protein binding tubulin binding identical protein binding poly(A) RNA binding fructose binding cadherin binding involved in cell-cell adhesion phosphatidylcholine binding ATPase binding fructose-1-phosphate aldolase activity protease binding molecular_function catalytic activity lyase activity aldolase

Process(es)

platelet degranulation fructose metabolic process gluconeogenesis glycolytic process ATP biosynthetic process striated muscle contraction actin filament organization regulation of cell shape fructose 1,6-bisphosphate metabolic process muscle cell cellular homeostasis protein homotetramerization canonical glycolysis cell-cell adhesion liver development NADH oxidation response to fructose response to zinc ion cellular response to extracellular stimulus positive regulation of ATPase activity cellular response to insulin stimulus response to drug response to starvation response to amino acid response to copper ion response to glucocorticoid response to cAMP fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate vacuolar proton-transporting V-type ATPase complex assembly response to interleukin-6 response to hypoxia response to heat response to lipopolysaccharide response to nicotine response to estrogen response to peptide hormone response to carbohydrate response to organic cyclic compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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