Invitrogen

Alpha-Smooth Muscle Actin Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

6个已发表图片

14 篇参考文献

查看其他44个Alpha-Smooth Muscle Actin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 12

Alpha-Smooth Muscle Actin Antibody (PA5-18292) in IHC (P)

Immunohistochemistry analysis of Alpha-Smooth Muscle Actin in human colon, smooth muscle. Samples were incubated with Alpha-Smooth Muscle Actin polyclonal antibody (Product # PA5-18292) using a dilution of 2.5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-18292

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-1 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Dog, Human, Mouse, Rat

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (EEEDSTALVC) corresponding to the N-terminus amino acids of ACTA2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_10980764

产品详细信息

This antibody is predicted to react with canine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

靶标信息

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

PA5-18292 was used in Western Blot, Immunohistochemistry to find an increased expression of the mesenchymal markers α-SMA and fibronectin in TFCs in the thyroid glands of AITD patients.

Wed Feb 08 00:00:00 EST 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

PA5-18292 was used in Western Blot, Immunohistochemistry to find an increased expression of the mesenchymal markers α-SMA and fibronectin in TFCs in the thyroid glands of AITD patients.

Wed Feb 08 00:00:00 EST 2023

Ciclopirox Inhibition of eIF5A Hypusination Attenuates Fibroblast Activation and Cardiac Fibrosis.

Journal of cardiovascular development and disease

Subbaiah KCV,Wu J,Tang WHW,Yao P

PA5-18292 was used in Western Blot, Immunohistochemistry to suggest that CPX can be repurposed to treat cardiac fibrosis and ischemic heart failure.

Sun Jan 29 00:00:00 EST 2023

DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice.

Communications biology

Senatus L,Egaña-Gorroño L,López-Díez R,Bergaya S,Aranda JF,Amengual J,Arivazhagan L,Manigrasso MB,Yepuri G,Nimma R,Mangar KN,Bernadin R,Zhou B,Gugger PF,Li H,Friedman RA,Theise ND,Shekhtman A,Fisher EA,Ramasamy R,Schmidt AM

PA5-18292 was used in Western Blot, Immunohistochemistry to unveil new regulators of atherosclerosis and lipid metabolism through DIAPH1.

Fri Mar 17 00:00:00 EDT 2023

DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice.

Communications biology

PA5-18292 was used in Western Blot, Immunohistochemistry to unveil new regulators of atherosclerosis and lipid metabolism through DIAPH1.

Fri Mar 17 00:00:00 EDT 2023

The generation of a lactate-rich environment stimulates cell cycle progression and modulates gene expression on neonatal and hiPSC-derived cardiomyocytes.

Biomaterials advances

Ordoño J,Pérez-Amodio S,Ball K,Aguirre A,Engel E

PA5-18292 was used in Immunocytochemistry to support a novel use of lactate in cardiac tissue engineering, modulating the metabolic environment of the heart and thus paving the way to the development of lactate-releasing platforms for in situ cardiac regeneration.

Mon Aug 01 00:00:00 EDT 2022

A uropathogenic E. coli UTI89 model of prostatic inflammation and collagen accumulation for use in studying aberrant collagen production in the prostate.

American journal of physiology. Renal physiology

Ruetten H,Sandhu J,Mueller B,Wang P,Zhang HL,Wegner KA,Cadena M,Sandhu S,L Abler L,Zhu J,O'Driscoll CA,Chelgren B,Wang Z,Shen T,Barasch J,Bjorling DE,Vezina CM

PA5-18292 was used in Immunohistochemistry to determine that this model is effective in altering urinary phenotype and producing prostatic inflammation and collagen accumulation in mice.

Fri Jan 01 00:00:00 EST 2021

Elastic Laminar Reorganization Occurs with Outward Diameter Expansion during Collateral Artery Growth and Requires Lysyl Oxidase for Stabilization.

Cells

McEnaney RM,McCreary DD,Skirtich NO,Andraska EA,Sachdev U,Tzeng E

PA5-18292 was used in Immunohistochemistry to show lysyl oxidase activity is necessary for arterial wall stabilization during outward remodeling.

Tue Dec 21 00:00:00 EST 2021

An immunohistochemical prostate cell identification key indicates that aging shifts procollagen 1A1 production from myofibroblasts to fibroblasts in dogs prone to prostate-related urinary dysfunction.

PloS one

Ruetten H,Cole C,Wehber M,Wegner KA,Girardi NM,Peterson NT,Scharpf BR,Romero MF,Wood MW,Colopy SA,Bjorling DE,Vezina CM

PA5-18292 was used in Immunohistochemistry to identify an aging-related shift in the prostatic cell type producing procollagen 1A1 that will help direct development of cell type and prostate appropriate therapeutics for collagen accumulation.

Tue Sep 08 00:00:00 EDT 2020

PloS one

Ruetten H,Cole C,Wehber M,Wegner KA,Girardi NM,Peterson NT,Scharpf BR,Romero MF,Wood MW,Colopy SA,Bjorling DE,Vezina CM

Tue Sep 08 00:00:00 EDT 2020

PloS one

Ruetten H,Cole C,Wehber M,Wegner KA,Girardi NM,Peterson NT,Scharpf BR,Romero MF,Wood MW,Colopy SA,Bjorling DE,Vezina CM

Tue Sep 08 00:00:00 EDT 2020

Nano-Polyplexes Mediated Transfection of Runx2-shRNA Mitigates the Osteodifferentiation of Human Valvular Interstitial Cells.

Pharmaceutics

Voicu G,Rebleanu D,Constantinescu CA,Fuior EV,Ciortan L,Droc I,Uritu CM,Pinteala M,Manduteanu I,Simionescu M,Calin M

PA5-18292 was used in Western Blotting to suggest that the silencing of Runx2 could represent a novel strategy to impede the osteoblastic phenotypic shift of VIC and the ensuing progress of CAVD.

Tue Jun 02 00:00:00 EDT 2020

Nano-Polyplexes Mediated Transfection of Runx2-shRNA Mitigates the Osteodifferentiation of Human Valvular Interstitial Cells.

Pharmaceutics

Voicu G,Rebleanu D,Constantinescu CA,Fuior EV,Ciortan L,Droc I,Uritu CM,Pinteala M,Manduteanu I,Simionescu M,Calin M

PA5-18292 was used in Western Blotting to suggest that the silencing of Runx2 could represent a novel strategy to impede the osteoblastic phenotypic shift of VIC and the ensuing progress of CAVD.

Tue Jun 02 00:00:00 EDT 2020

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.

Cell

Zhao Q,Liu J,Deng H,Ma R,Liao JY,Liang H,Hu J,Li J,Guo Z,Cai J,Xu X,Gao Z,Su S

PA5-18292 was used in Immunohistochemistry (Paraffin) to identify a mitochondrial circRNA that drives metaflammation and serves as a therapeutic target for NASH.

Thu Oct 01 00:00:00 EDT 2020

The Oxysterol Synthesising Enzyme CH25H Contributes to the Development of Intestinal Fibrosis.

Journal of Crohn's & colitis

Raselli T,Wyss A,Gonzalez Alvarado MN,Weder B,Mamie C,Spalinger MR,Van Haaften WT,Dijkstra G,Sailer AW,Imenez Silva PH,Wagner CA,Tosevski V,Leibl S,Scharl M,Rogler G,Hausmann M,Misselwitz B

Published figure using Alpha-Smooth Muscle Actin polyclonal antibody (Product # PA5-18292) in Western Blot

Thu Sep 19 00:00:00 EDT 2019

mTORC1 amplifies the ATF4-dependent de novo serine-glycine pathway to supply glycine during TGF-β₁-induced collagen biosynthesis.

Science signaling

Selvarajah B,Azuelos I,Platé M,Guillotin D,Forty EJ,Contento G,Woodcock HV,Redding M,Taylor A,Brunori G,Durrenberger PF,Ronzoni R,Blanchard AD,Mercer PF,Anastasiou D,Chambers RC

PA5-18292 was used in Immunohistochemistry to show that TGF-β1-induced ATF4 production dependes on cooperation between canonical TGF-β1 signaling through Smad3 and activation of mechanistic target of mTORC1and its downstream target eukaryotic translation initiation factor 4E-BP1.

Tue May 21 00:00:00 EDT 2019

Combination Growth Factor Therapy via Electrostatically Assembled Wound Dressings Improves Diabetic Ulcer Healing In Vivo.

Advanced healthcare materials

Almquist BD,Castleberry SA,Sun JB,Lu AY,Hammond PT

PA5-18292 was used in immunohistochemistry to calculate an improvement in diabetic ulcer healing in vivo by combination growth factor therapy via electrostatically assembled wound dressings

Thu Oct 01 00:00:00 EDT 2015

生物信息学

蛋白别名: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha Cardiac Actin; Alpha-actin-2; alpha-cardiac actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin

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基因别名: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA

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UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737

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Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475

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功能

ATP binding protein kinase binding nucleotide binding protein binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

参与通路

muscle contraction regulation of blood pressure response to virus positive regulation of gene expression vascular smooth muscle contraction glomerular mesangial cell development mesenchyme migration