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AmCyan-Tag Antibody (BSM-33184M) in WB
Lane 1:AmCyan-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33184P);Lane 2: Control (-) E.coli lysates probed with AmCyan-Tag Monoclonal Antibody, Unconjugated (bsm-33184M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
产品信息
BSM-33184M
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
5F9
抗原
Recombinant AmCyan
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
TBS, pH 7.4, with 1% BSA, 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AmCyan1; cyan fluorescent
