Invitrogen

ApoA1 Polyclonal Antibody, PeproTech®

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产品信息

500-P331-1MG

应用
建议稀释比
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免疫印迹 (WB)

0.1-0.2 µg/mL
-

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL
-
产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 28.2 kDa Recombinant Human ApoA-I
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930463

产品详细信息

AA Sequence of recombinant protein: MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ApoA-I. Anti-Human ApoA-I-specific antibody was purified by affinity chromatography employing an immobilized Human ApoA-I matrix.

Sandwich ELISA: To detect Human ApoA-I by sandwich ELISA (using 100 µL/well), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ApoA-I (500-P331BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human ApoA-I.

Western Blot: To detect Human ApoA-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human ApoA-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; Udpgt; Ugt1; Ugt1a7

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基因别名: apo(a); APOA1

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UniProt ID: (Human) P02647

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Entrez Gene ID: (Human) 335

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功能
beta-amyloid binding protein binding phospholipid binding phospholipid transporter activity high-density lipoprotein particle binding cholesterol binding cholesterol transporter activity enzyme binding phosphatidylcholine binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity lipase inhibitor activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding apolipoprotein
参与通路
retinoid metabolic process regulation of protein phosphorylation endothelial cell proliferation platelet degranulation negative regulation of cytokine secretion involved in immune response phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process receptor-mediated endocytosis transforming growth factor beta receptor signaling pathway G-protein coupled receptor signaling pathway integrin-mediated signaling pathway response to nutrient cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling peripheral nervous system axon regeneration protein oxidation peptidyl-methionine modification triglyceride catabolic process lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development organ regeneration neuron projection regeneration regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion high-density lipoprotein particle remodeling high-density lipoprotein particle assembly high-density lipoprotein particle clearance positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process response to drug cholesterol homeostasis blood vessel endothelial cell migration response to estrogen reverse cholesterol transport cellular protein metabolic process positive regulation of fatty acid biosynthetic process negative regulation of interleukin-1 beta secretion negative regulation of inflammatory response protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport positive regulation of hydrolase activity positive regulation of stress fiber assembly transmembrane transport phospholipid homeostasis negative regulation of lipase activity negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis ERK1 and ERK2 cascade cholesterol import positive regulation of substrate adhesion-dependent cell spreading
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