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ApoA1 Antibody (MA5-14732) in IHC (P)
Immunohistochemistry was performed on human liver tissue sections. To expose Apo A-1, tissues were microwaved for 10 minutes in 10mM sodium citrate, pH 6.0. Following antigen retrieval, endogenous peroxidases were blocked in a 3% hydrogen peroxide-methanol solution for 15 minutes, and then tissues were blocked in 3% BSA in PBS for 30 minutes at room temperature. Tissues were probed with an Apo A-1 monoclona... View More
产品信息
MA5-14732
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
532
抗原
Purified human plasma Apo A-1
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11009136
产品详细信息
By sandwich ELISA, MA5-14732 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14732, a biotinylated detection antibody followed by Streptavidin-HRP is recommended. By Western blot, MA5-14732 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14732 is also recommended for detecting endogenous Apo A-1 by Western blot.
By Western blot, MA5-14732 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14732 is also recommended for detecting endogenous Apo A-1 by Western blot.
Product MA514732 is a smaller package size of MIA1405 (formerly sold as a Seradyn product).
靶标信息
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; Udpgt; Ugt1; Ugt1a7
基因别名: apo(a); APOA1
UniProt ID: (Human) P02647
Entrez Gene ID: (Human) 335
